首页|Diagnostic Value of Targeted Next-generation Sequencing in Pulmonary Mycobacterial Infections
Diagnostic Value of Targeted Next-generation Sequencing in Pulmonary Mycobacterial Infections
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Objective:This study aimed to explore the diagnostic value of novel technique-targeted next-generation sequencing(tNGS)of bronchoalveolar lavage fluid(BALF)in pulmonary mycobacterial infections.Methods:This retrospective study was conducted on patients who underwent bronchoscopy and tNGS,smear microscopy,and mycobacterial culture of BALF.Patients with positive Mycobacterium tuberculosis(MTB)culture or GeneXpert results were classified into the tuberculosis case group.Those diagnosed with nontuberculous mycobacteria(NTM)-pulmonary disease(NTM-PD)composed the case group of NTM-PD patients.The control group comprised patients without tuberculosis or NTM-PD.Sensitivity,specificity,and receiver operating characteristic(ROC)curves were used to evaluate the diagnostic performance.Results:For tuberculosis patients with positive mycobacterial culture results,the areas under the ROC curves(AUCs)for tNGS,GeneXpert,and smear microscopy were 0.975(95％CI:0.935,1.000),0.925(95％CI:0.859,0.991),and 0.675(95％CI:0.563,0.787),respectively.For tuberculosis patients with positive GeneXpert results,the AUCs of tNGS,culture,and smear microscopy were 0.970(95％CI:0.931,1.000),0.850(95％CI:0.770,0.930),and 0.680(95％CI:0.579,0.781),respectively.For NTM-PD,the AUCs of tNGS,culture,and smear-positive but GeneXpert-negative results were 0.987(95％CI:0.967,1.000),0.750(95％CI:0.622,0.878),and 0.615(95％CI:0.479,0.752),respectively.The sensitivity and specificity of tNGS in NTM-PD patients were 100％and 97.5％,respectively.Conclusion:tNGS demonstrated superior diagnostic efficacy in mycobacterial infection,indicating its potential for clinical application.
Department of Respiratory and Critical Care Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
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NSTL
万方数据
