首页|Electroacupuncture Alleviates Memory Deficits in APP/PS1 Mice by Targeting Serotonergic Neurons in Dorsal Raphe Nucleus

Electroacupuncture Alleviates Memory Deficits in APP/PS1 Mice by Targeting Serotonergic Neurons in Dorsal Raphe Nucleus

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Objective:Alzheimer's disease(AD)has become a significant global concern,but effective drugs able to slow down AD progression is still lacked.Electroacupuncture(EA)has been demonstrated to ameliorate cognitive impairment in individuals with AD.However,the underlying mechanisms remains poorly understood.This study aimed at examining the neuroprotective properties of EA and its potential mechanism of action against AD.Methods:APP/PS1 transgenic mice were employed to evaluate the protective effects of EA on Shenshu(BL 23)and Baihui(GV 20).Chemogenetic manipulation was used to activate or inhibit serotonergic neurons within the dorsal raphe nucleus(DRN).Learning and memory abilities were assessed by the novel object recognition and Morris water maze tests.Golgi staining,western blot,and immunostaining were utilized to determine EA-induced neuroprotection.Results:EA at Shenshu(BL 23)and Baihui(GV 20)effectively ameliorated learning and memory impairments in APP/PS1 mice.EA attenuated dendritic spine loss,increased the expression levels of PSD95,synaptophysin,and brain-derived neurotrophic factor in hippocampus.Activation of serotonergic neurons within the DRN can ameliorate cognitive deficits in AD by activating glutamatergic neurons mediated by 5-HT1B.Chemogenetic inhibition of serotonergic neurons in the DRN reversed the effects of EA on synaptic plasticity and memory.Conclusion:EA can alleviate cognitive dysfunction in APP/PS1 mice by activating serotonergic neurons in the DRN.Further study is necessary to better understand how the serotonergic neurons-related neural circuits involves in EA-induced memory improvement in AD.

Alzheimer's diseaseelectroacupuncturedorsal raphe nucleushippocampusserotonergic neuronsglutamatergic neurons5-HT1Bcognitive impairmentchemogenetic manipulationsynaptic plasticity

Chao-chao YU、Xiao-fei WANG、Jia WANG、Chu LI、Juan XIAO、Xue-song WANG、Rui HAN、Shu-qin WANG、Yuan-fang LIN、Li-hong KONG、Yan-jun DU

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Department of Rehabilitation,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China

Department of Tuina,Shenzhen Traditional Chinese Medicine Hospital,Shenzhen 518000,China

The 4th Clinical College,Guangzhou University of Chinese Medicine,Shenzhen 518000,China

Department of Rehabilitation,Wuhan Third Hospital,Tongren Hospital of Wuhan University,Wuhan 430074,China

Department of Acupuncture and Moxibustion,Wuhan Hospital of Integrated Traditional Chinese and Western Medicine,Wuhan 430030,China

College of Acupuncture and Orthopedics,Hubei University of Chinese Medicine,Wuhan 430065,China

College of Acupuncture-Moxibustion and Tuina,Hebei University of Chinese Medicine,Shijiazhuang 050299,China

Department of Child Rehabilitation Medicine,Qujing Hospital of Maternity and Childcare,Qujing 655002,China

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2024

10.1007/s11596-024-2908-9

当代医学科学(英文)
华中科技大学同济医学院

当代医学科学(英文)

影响因子：0.748
ISSN：2096-5230
年,卷(期)：2024.44(5)