首页|Kaempferol Improved Rheumatoid Arthritis by Regulating the Immune Imbalance of Treg/Th17

Kaempferol Improved Rheumatoid Arthritis by Regulating the Immune Imbalance of Treg/Th17

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Objective:The objective of this study was to explore the therapeutic effects of kaempferol(Kae)on rheumatoid arthritis(RA)and to elucidate the underlying mechanisms.Methods:The collagen-induced arthritis(CIA)model was established using collagen Ⅱ to induce RA.Mice were treated with Kae at a dose of 25 or 50 mg/kg/day via gavage.Pathological changes in the ankle joint were analyzed.Enzyme-linked immunosorbent assay(ELISA)was employed to measure the levels of inflammatory factors.Reverse transcription quantitative polymerase chain reaction(RT-qPCR)was used to assess the expression of genes associated with the balance of regulatory T(Treg)/T helper 17(Th 17)cells.Flow cytometry was utilized to determine the Treg/Th17 ratio.Furthermore,these techniques were employed to evaluate the impact of miR-34a and Foxp3 dysregulation on cellular functions in RA under the influence of Kae.Dual luciferase reporter assay was conducted to analyze the binding of miR-34a to Foxp3.Results:Treatment with Kae led to a downregulation of receptor-related orphan receptor gamma t(RORγt)and IL-17 expression,and an upregulation of Foxp3,IL-10,and TGF-β expression in CIA mice.Kae intervention inhibited the production of proinflammatory cytokines and increased the production of anti-inflammatory cytokines.Furthermore,Kae treatment suppressed the expression of miR-34a,which was identified as a target of miR-34a.Finally,Kae regulated Treg/Th 17 balance-related genes and cellular inflammation through the miR-34a/Foxp3 axis.Conclusion:The study demonstrated that Kae effectively ameliorates CIA in mice by modulating the Treg/Th17 balance and related genes via the miR-34a/Foxp3 axis.These findings suggest that Kae may serve as a promising therapeutic agent for the treatment of RA and for restoring immune homeostasis.

rheumatoid arthritiskaempferolmiR-34aTreg/Th17Foxp3

Nan LI、Yan-kui YI、Jie ZHAO、Qiang WANG、Jie-ying YU、Yan-ting YOU、Yong-yan ZHU、Yan-yan LIU、Xiao-shan ZHAO、Dong-mei PAN

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Department of Clinical Basis of Traditional Chinese Medicine,School of Traditional Chinese Medicine,Jinan University,Guangzhou 510632,China

Department of Laboratory of Chinese Medicine Pharmaceutics,School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China

Health College of Guangdong Pharmaceutical University,Guangzhou 510006,China

Department of Traditional Chinese Medicine,People's Hospital of Yangjiang,Guangzhou 529500,China

Department of Clinical Basis of Traditional Chinese Medicine,School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China

Department of Syndrome Laboratory of Integrated Chinese and Western Medicine,School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China

Department of Chemistry and Analysis of Traditional Chinese Medicine,School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China

Department of Internal Medicine of Traditional Chinese Medicine,School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China

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2024

10.1007/s11596-024-2925-8

当代医学科学(英文)
华中科技大学同济医学院

当代医学科学(英文)

影响因子：0.748
ISSN：2096-5230
年,卷(期)：2024.44(6)