摘要
目的 探讨LINC00115靶向miR-874-3p对肝癌细胞生物学行为和紫杉醇敏感性的影响.方法 qRT-PCR检测 LINC00115 和 miR-874-3p 在肝癌组织、细胞系中的表达情况.将 si-NC、si-LINC00115、miR-NC、miR-874-3p、pcD-NA、pcDNA-LINC00115、anti-miR-NC+si-LINC00115、anti-miR-874-3p+si-LINC00115 分别转染肝癌细胞 MHCC97H.采用CCK-8法测定细胞活力和对紫杉醇的IC5.值.Transwell实验检测细胞迁移和侵袭.双荧光素酶报告基因实验确定LINC00115和miR-874-3p的关系.结果 肝癌组织、细胞系中LINC00115呈高表达(均P＜0.05),miR-874-3p呈低表达(均P＜0.05).下调LINC00115表达后细胞吸光度(A)值、对紫杉醇IC5.值、迁移和侵袭显著降低(均P＜0.05),而miR-874-3p表达显著增加(均P＜0.05).上调miR-874-3p表达后细胞A值、对紫杉醇IC5.值、迁移和侵袭显著减少(均P＜0.05).上调 LINC00115 表达后 miR-874-3p 表达显著减少(P＜0.05).LINC00115 与 miR-874-3p 直接结合.下调miR-874-3p表达显著减弱LINC00115对肝癌细胞A值、对紫杉醇IC50值、迁移和侵袭的影响(均P＜0.05).结论 下调LINC00115通过促进miR-874-3p表达来抑制肝癌细胞增殖、迁移和侵袭行为,增加其对紫杉醇的敏感性.
Abstract
Objective To explore the effect of LINC00115 targeting miR-874-3p on the biological behavior and paclitaxel sensitivity of liver cancer cells.Methods The qRT-PCR was performed to detect the expression levels of LINC00115 and miR-874-3p in liver cancer tissues and cell lines.The si-NC,si-LINC00115,miR-NC,miR-874-3p,pcDNA,pcDNA-LINC00115,anti-miR-NC+si-LINC00115,anti-miR-874-3p+si-LINC00115 were transfected into liver cancer cells MHCC97H respective-ly.CCK-8 method was applied to assess cell viability and IC50 value to paclitaxel.Transwell assay was performed to detect cell migration and invasion.Dual luciferase reporter gene method was used to determine the relationship between LINC00115 and miR-874-3p.Results LINC00115 was highly expressed(P＜0.05),while miR-874-3p was lowly expressed(P＜0.05)in liver cancer tissues and cell lines.After downregulating LINC00115,the cell absorbance(A)value,the IC50 value to paclitaxel,migra-tion and invasion were significantly reduced(all P＜0.05),while miR-874-3p expression was significantly increased(P＜0.05).After upregulating miR-874-3p,the cell A value,IC50 value to paclitaxel,migration and invasion were significantly reduced(all P＜0.05).After upregulating LINC00115,miR-874-3p expression was decreased(P＜0.05).LINC00115 had a direct interaction with miR-874-3p.Downregulating miR-874-3p significantly reduced the effect of low LINC00115 expression on A value,IC50 value to paclitaxel,migration and invasion of liver cancer cells(all P＜0.05).Conclusion Downregulation of LINC00115 inhibits the prolifera-tion,migration and invasion of liver cancer cells to increase paclitaxel sensitivity by promoting miR-874-3p expression.
基金项目
湖北省自然科学基金资助项目(2022CFB980)
武汉市卫健委医学科研项目(WX21D48)
维普
万方数据