Comparison of Electrocoagulation and Filament Occlusion in Construction of Middle Cerebral Artery Ischemia Model for Central Post-Stroke Pain Study
Objective To induce middle cerebral artery ischemia by electrocoagulation or filament occlusion to construct central post-stroke pain(CPSP)model in mice,and to identify a more valid and producible animal model of CPSP.Methods MaleC57BL/6 mice were randomly divided into control(Naive)group,electrocoagulation(dMCAO)group and filament(tM-CAO)group.Neurological defects of mice were measured by using the Longa scale.TTC staining was used to evaluate brain in-farct volume.Paw withdrawal threshold and thermal withdrawal latency were used to evaluate nociceptive behavior.Open field test was used to assess motor function.Results Compared with Naive group,the neurological defect scores of both dMCAO and tM-CAO group were increased.TTC staining showed considerable discrepancies of infarct area between dMCAO and tMCAO group.Compared with Naive group,mice in both dMCAO and tMCAO group developed mechanical allodynia and thermal hyperalgesia on day 7,14,21,28 after surgery.Furthermore,locomotor activity was not altered on day 29 in dMCAO group as compared with the Na-ive group,while that of tMCAO group was significantly declined.Conclusion Both dMCAO and tMCAO model can lead to pain-related syndrome,but dMCAO model is more reproducible and capable to mimic clinical characters of CPSP.
central post-stroke painanimal modelneuropathic painmiddle cerebral artery occlusionbrain ischemia
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