The Expression of PMCA1-4 and Its Splice Variants at Sites A and C in Vestibular Tissue of Adult Rats
Objective To detect the distribution sites of plasma membrane Ca2+-ATPase 1-4(PMCA1-4)and the types of splice variants at sites A and C in adult rat vestibular tissues.Methods Healthy SD rats eight-week old were selected.The inner ear organs were dissected for vestibular sections,while vestibular macula sacculi and macula utriculi were isolated for total RNA extraction.The expression of PMCA1-4 in vestibular tissues of adult rat inner ear was detected by immunofluorescence,and the expression of A-terminal and C-terminal splice variants of PMCA1-4 in vestibular tissues of adult rat was detected by reverse transcription-polymerase chain reaction method.Results PMCA1 was expressed in the basolateral membrane of hair cells and supporting cells in saccule and utricle.PMCA2 was strongly expressed in the hair cell bundles of saccule and utricle.PMCA3 was weakly expressed in the basolateral membrane of hair cells and supporting cells in saccule and utricle.PMCA4 was poorly expressed in saccule and utricle.The four PMCA isoforms respectively expressed PMCA1x/b,PM-CA2w/b,PMCA3z/(a,b)and PMCA4x/b in the saccule,their expression pattern in the utricle is the same as that in the sac-cule.Conclusion The distribution sites of PMCA1-4 varied in the vestibular organs of adult rats,and the spilce variants of the four PMCA subtypes were also variant.The distribution diversity is likely to associated with the special demands of Ca2+regu-lation in vestibular tissues and subcellular domains.
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