Spag6 Alleviated Brain Edema Injury Caused by Cerebral Ischemia-Reperfusion in Rats by Regulating Motor Cilia Structure and Function
Objective To investigate the effect of sperm-associated antigen 6(Spag6)on cerebral edema secondary to ische-mia-reperfusion in rats.Methods Ninety SD rats were randomly assigned to three groups:sham operation group,model group,and experimental group.The rats were treated with lentivirus injection and middle cerebral artery embolization.The infarct vol-ume was evaluated by using 2,3,5-triphenyltetrazolium chloride(TTC)staining.Hematoxylin and eosin(HE)staining was used to observe the damage of ependymal cells and cilia.Scanning electron microscope was used to observe the activity of motile cili-a.Transmission electron microscopy was used to abserve the ultrastructure of nerve cells.The expressions of apoptosis-related proteins Bcl-2,Bax,Survivin,GluN1,GluN2B,and GluA2 were detected using Western blotting.Results After model construc-tion,nerve cells and tissues in different regions of the rat brain were damaged.Spag6 expression,synaptic structure and function were altered.The activity of motor cilia in the third ventricle was disturbed and ischemic cerebral edema was observed.After up-regulation of Spag6 expression,cerebral edema was relieved and the activity of motor cilia was restored.Ischemic cerebral infarc-tion,nerve cell injury and synaptic structural abnormalities were reversed.The protein expression of Bcl-2,Survivin,GluN1,GluN2B,and GluA2 increased significantly(P＜0.01 or P＜0.05),while the expression of Bax decreased(P＜0.01).Conclusion Spag6 enhances synaptic function by restoring the active state of ventricular motor cilia,thereby alleviating ischemic brain edema mediated nerve damage.
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