Association of MTHFR and MTRR Gene Mutations with Coronary Artery Stenosis in Patients with Coronary Heart Disease
Objective To analyze the association of 5,10-methylene tetrahydrofolate reductase(MTHFR)and methionine synthase reductase(MTRR)gene mutations with the severity of coronary artery stenosis in coronary heart disease pa-tients.Methods In all,135 coronary heart disease patients admitted to cardiology department of People's Hospital of Hulunbuir from June 2019 to June 2021 were selected as the study subjects,and the patients were divided into control group(Gensini score＜30 points)and observation group(Gensini score≥ 30 points).The polymorphism of MTHFR677,MTHFR1298 and MTRR66 gene was detected by real-time fluorescence quantitative polymerase chain reaction,and the gene mutation was compared be-tween two groups.The correlation between gene polymorphism,serum Hcy level and Gensini score was analyzed.Results There were three genotypes(CC,CT and TT)for MTHFR gene 677,three genotypes(AA,AC and CC)for MTHFR gene 1298,and three genotypes(AA,AG and GG)for MTRR gene 66.The T allele frequency(67.81％vs.54.03％)and TT genotype fre-quency(46.58％vs.25.81％)of MTHFR677 locus in the observation group were higher than those in the control group.The C allele frequency(11.64％vs.22.58％)and CC genotype frequency(0.00％vs.9.68％)of MTHFR1298 locus in the observation group were lower than those in the control group(P＜0.05).There was no significant difference in the allele frequency and gen-otype frequency of MTRR locus 66 between the two groups(P＞0.05).There was a statistically significant difference in serum Hcy levels among patients with MTHFR677 and MTHFR1298 genotypes.Among them,TT genotype in MTHFR677 gene was significantly higher than CC and CT,while AC+CC genotype in MTHFR1298 gene was significantly lower than AA genotype(all P＜0.05).There was no significant difference in serum Hcy levels among MTRR66 gene genotypes(P＞0.05).Multiple linear regression analysis showed that MTHFR677 was positively correlated with Gensini score,MTHFR1298 was negatively correlated with Gensini score(P＜0.05),and MTRR66 was not significantly correlated with Gensini score(P＞0.05).Conclusion MT H FR gene C677 T and A1298 C mutations are strongly associated with severity of coronary artery stenosis,which could be potential predictive indicators for coronary heart disease.
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