摘要
目的 探究枸杞多糖(LBP)通过调控细胞铁死亡对胆囊癌细胞(NOZ和SGC-996)增殖和转移的影响.方法 不同剂量的LBP处理体外培养NOZ和SGC-996细胞;将细胞分为对照组(Control)、20 μmol/L LBP组、40 μmol/L LBP组、60 μmol/L LBP组、80 μmol/L LBP组、LBP+Fer-1组.用MTT法检测细胞活力;EdU法检测细胞增殖能力;Tran-swell检测细胞侵袭能力;比色法检测Fe2+的水平,BODIPYTM 581/591 C11分子探针测定活性氧(ROS)和丙二醛(MDA)的水平;Western blot检测铁死亡相关蛋白谷胱甘肽过氧化物酶4(GPX4)酰基辅酶a合成酶长链家族成员4(ACSL4)、β-catenin和Wnt3A蛋白表达.结果 与对照组比较,40 μmol/L LBP组、60 μmol/L LBP组细胞活力、增殖、侵袭能力显著降低(均P＜0.05),Fe2+水平、ROS、MDA活性明显增加(均P＜0.05),GPX4、β-catenin和Wnt3A蛋白表达显著减少,ACSL4蛋白表达明显增加(均P＜0.05).与LBP组比较,LBP+Fer-1组细胞活力、增殖、侵袭能力显著提高(均P＜0.05).结论 LBP通过诱导胆囊癌细胞铁死亡进而抑制其细胞的增殖和转移.
Abstract
Objective To investigate the effects of lycium barbarum polysaccharide(LBP)on the proliferation and metastasis of gallbladder cancer cells(NOZ and SGC-996)by regulating cellular ferroptosis.Methods NOZ and SGC-996 cells were cul-tured in vitro by treatment with different doses of LBP.The cells were divided into control group,20 μmol/L LBP group,40μmol/L LBP group,60 μmol/L LBP group,80 μmol/L LBP group,and LBP+Fe-1 groups.Cell activity was detected by MTT assay.Cell proliferation was detected by EdU assay.Cell invasion ability was detected by Transwell assay.Fe2+level was detec-ted by colorimetric assay,reactive oxygen species(ROS)and malondialdehyde(MDA)levels were determined by BODIPYT 581/591 C11 molecular probe.Western blot was used to detect glutathione peroxidase 4(GPX4),acyl-CoA Synthetase long-chain family member 4(ACSL4),β-catenin,and Wnt3A protein expression.Results Compared with the control group,cell viability,proliferation,and invasion were significantly decreased in the 40 μmol/L LBP,and 60 μmol/L LBP groups(all P＜0.05),and Fe2+level,ROS,and MDA activities were significantly increased(all P＜0.05),and GPX4,β-catenin,and Wnt3A protein ex-pressions were significantly reduced,and ACSL4 protein expression was significantly increased(all P＜0.05).Compared with the LBP group,cell viability,proliferation,and invasion ability were significantly increased in the LBP+Fer-1 group(all P＜0.05).Conclusion LBP inhibits the proliferation and metastasis of gallbladder cancer cells by inducing ferroptosis.
基金项目
宁夏回族自治区自然科学基金资助项目(2023AAC03506)
维普
万方数据