Lycium Barbarum Polysaccharide Inhibits the Malignant Progression of Gallbladder Cancer Cells by Promoting Cellular Ferroptosis
Objective To investigate the effects of lycium barbarum polysaccharide(LBP)on the proliferation and metastasis of gallbladder cancer cells(NOZ and SGC-996)by regulating cellular ferroptosis.Methods NOZ and SGC-996 cells were cul-tured in vitro by treatment with different doses of LBP.The cells were divided into control group,20 μmol/L LBP group,40μmol/L LBP group,60 μmol/L LBP group,80 μmol/L LBP group,and LBP+Fe-1 groups.Cell activity was detected by MTT assay.Cell proliferation was detected by EdU assay.Cell invasion ability was detected by Transwell assay.Fe2+level was detec-ted by colorimetric assay,reactive oxygen species(ROS)and malondialdehyde(MDA)levels were determined by BODIPYT 581/591 C11 molecular probe.Western blot was used to detect glutathione peroxidase 4(GPX4),acyl-CoA Synthetase long-chain family member 4(ACSL4),β-catenin,and Wnt3A protein expression.Results Compared with the control group,cell viability,proliferation,and invasion were significantly decreased in the 40 μmol/L LBP,and 60 μmol/L LBP groups(all P＜0.05),and Fe2+level,ROS,and MDA activities were significantly increased(all P＜0.05),and GPX4,β-catenin,and Wnt3A protein ex-pressions were significantly reduced,and ACSL4 protein expression was significantly increased(all P＜0.05).Compared with the LBP group,cell viability,proliferation,and invasion ability were significantly increased in the LBP+Fer-1 group(all P＜0.05).Conclusion LBP inhibits the proliferation and metastasis of gallbladder cancer cells by inducing ferroptosis.
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