Effect and Mechanism of OMA1 Gene on Proliferation,Migration,Invasion and Apoptosis of Human Lung Cancer Cell
Objective To determine the expression of OMA1 gene in human lung cancer cells A549.To study its effects on cell proliferation,migration,invasion and apoptosis of human lung cancer cell A549 and to explore its action mechanism.Methods The gene and protein expression of OMA1 in human lung cancer cells and normal lung epithelial cells was detected.After Calu-3 cells with the highest expression of OMA1 were transfected with OMA1 gene interfering lentivirus(shOMA1)and A549 cells with the lowest expression of OMA1 were transfected with OMA1 gene overexpression lentivirus(oeOMA1),proliferation,mi-gration,invasion,apoptosis rate,and mRNA and protein expression of PI3K/AKT signaling pathway in each group were deter-mined.Results The gene and protein expression of OMA1 in human lung cancer cells was increased significantly compared to that of normal epithelial cells.The gene and protein expression of OMA1 in Calu-3 cells was the highest,and in A549 cells was the lowest.After Calu-3 cells were transfected with shOMA1,the expression level of OMA1 gene and protein decreased signifi-cantly.The proliferative ability,migrative ability,and the number of invasive cells increased significantly.Cell apoptosis rate,mRNA and protein expression of PI3K/AKTsignaling pathway decreased significantly.After A549 cells were transfected with oeOMA1,the gene and protein expression of OMA1 increased significantly.The proliferative ability,migrative ability,and the number of invasive cells decreased significantly.The apoptosis rate,mRNA and protein expression of PI3K/AKT signaling pathway increased significantly.Conclusion The expression of OMA1 is low in lung cancer cells.OMA1 may inhibit the prolif-eration,migration,invasion and apoptosis of lung cancer cells by inhibiting the expression of genes and proteins in PI3K/AKT signaling pathway.
OMA1lung cancercell proliferationcell migrationcell invasioncell apoptosisPI3K/AKT signal pathway
万方数据
维普
