Effect of Chrysophanol on Neurological Function in Severe Traumatic Brain Injury Rats Based on the NF-κB/ICAM-1 Pathway
Objective To investigate the improvement effect of chrysophanol(Chr)on neurological function in rats with se-vere traumatic brain injury(sTBI)based on the nuclear factor-κB(NF-κB)/intercellular adhesion molecule-1(ICAM-1)path-way.Methods Rats were separated into sTBI group,control group,Chr low-dose group,Chr high-dose group,edaravone group,Chr high-dose+NF-κB agonist lipopolysaccharide(LPS)group,with 18 rats in each group.Except for the control group,rats in all other groups were used to construct sTBI models using the improved Freeney's free fall method.Drugs were given once a day for 7 days after modeling for 6 hours.The modified neurological deficit score(mNSS),escape latency,number of platform crossing,and changes in brain water content were detected in rats.HE staining was applied to detect pathological changes in brain tissue.ELISA was applied to detect levels of interleukin(IL)-6,IL-12,and tumor necrosis factor-α(TNF-α)in brain tis-sue.TUNEL staining was applied to detect apoptosis in brain tissue cells.Western blot was applied to detect Bcl-2-associated X protein(Bax),Caspase-9,p-NF-κB p65,and ICAM-1 protein in brain tissue.Results Compared with the control group,severe brain tissue damage of the sTBI group rats,the mNSS score,brain water content,levels of IL-6,IL-12,TNF-α in brain tissue,cell apoptosis rate,and Bax,Caspase-9,p-NF-κB p65,ICAM-1 proteins increased,the escape latency extended,the number of platform crossing decreased(all P＜0.05).Compared with the sTBI group,the brain tissue cell morphology in the Chr low-dose,Chr high-dose group,and edaravone group improved.The mNSS score,brain water content,levels of IL-6,IL-12,TNF-α in brain tissue,cell apoptosis rate,and Bax,Caspase-9,p-NF-κB p65,ICAM-1 proteins decreased,the escape latency shortened,the number of platform crossing increased(all P＜0.05).LPS could reverse the effect of high-dose Chr on sTBI rats.Conclusion The mechanism by which chrysophanol improves neurological function,inhibits neuroinflammation and cell apoptosis in sTBI rats may be related to the downregulation of the NF-κB/ICAM-1 pathway.
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维普
