Objective To study the effects of muscone on the behavior and neuroinflammation of Tourette syndrome(TS)rats,and its related mechanisms.Methods Sixty rats were randomly divided into control group(Control group),model group(TS group),low-dose muscone group(L-Mus group),high-dose muscone group(H-Mus group)and positive drug group(Tia-pride group),with 12 rats in each group.Rats in Control group were intraperitoneally injected with normal saline,and rats in other groups were intraperitoneally injected with 3,3-iminodipropanitrile(IDPN)to establish TS rat model.The behavior of rats was measured by double-blind method.The levels of 5-hydroxytryptamine(5-HT),dopamine(DA),interleukin-6(IL-6),inter-leukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)were measured by ELISA,the protein expression was detected by Western blot and immunohistochemistry.Results Compared with Control group,the scores of exercise behavior and stereotyped behav-ior of rats in TS group were increased.The levels of IL-6,IL-1β and TNF-α in serum and striatum were increased,and the levels of 5-HT and DA in striatum were decreased.The protein expression of brain-derived neurotrophic factor(BDNF)was de-creased.The protein expressions of phosphorylated nuclear factor kappa B p65(p-NF-κB p65)and phosphorylated NF-κB inhibi-tory protein α(p-IKBα)were increased(all P＜0.05).Compared with TS group,the levels of the above indicators of rats in L-Mus group,H-Mus group and Tiapride group were significantly improved,and the improvement degree of all indicators level of rats in H-Mus group and Tiapride group were significantly better than that in L-Mus group(all P＜0.05).Conclusion Mus-cone can improve the behavior and neuroinflammation condition of TS rats,and its mechanism may be related to the regulation of BDNF/NF-κB signaling pathway.
维普
万方数据