Study on the chemical composition and antioxidant activity of Yangxue Qingnao alcohol extract
Objective:To study the chemical components and antioxidant activity of Yangxue Qingnao alcohol extract.Methods:Chromatographic separation techniques such as D101 macroporous resin column chromatography,silica gel column chromatography,octadecylsilyl(ODS)column chromatography,and preparative high performance liquid chromatography(HPLC)for separation and purification.Spectral techniques such as mass spectrometry and nuclear magnetic resonance spectroscopy were used to identify the structure of the compound,and the antioxidant activity of the monomer compound was investigated through 1,1-diphenyl-2-picrylhydrazine(DPPH)radical scavenging test.Results:Nineteen compounds were isolated and identified from the alcohol extract of Yangxue Qingnao,which were identified as glucose obtusin(1),gluco-obtusifolin(2),aurantio-obtusin(3),senkyunolide I(4),senkyunolide A(5),sedanolide(6),obtusifolin(7),chuanxiongnolide R1(8),senkyunolide N(9),senkyunolide J(10),E-6,7-trans-dihydroxyligustilide(11),chryso-obtusin-2-O-β-D-glucoside(12),question(13),chrysoobtusin(14),obtusin(15),chrysophanol(16),senkyunolide H(17),Z-6-hydroxy-7-methoxy-glucilide(18),3-butylidene-6α-hydroxy-7α-ethoxyphthalide(19).Conclusions:The chemical composition and antioxidant activity of the brain alcohol extract from nourishing serum were studied for the first time.The isolated compounds all have certain antioxidant activity,among which senkyunolide I has good antioxidant activity.
alcohol extract of Yangxue Qingnaoprescriptionmaterial baseantioxidant ability
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