Research on the role of cannabinoid receptor 1 in hepatic stellate cell activation and interfering effects of Haobie Yangyin Ruanjian
Objective:To explore the role of cannabinoid receptor1 in the activation of hepatic stellate cells(HSCs)and interfering effects of Haobie Yangyin Ruanjian.Methods:Human hepatic stellate cells LX-2 with immortalized characteristics were used as the research object,and the experiment was divided into LX-2 cell control group,HBYYRJ group,CBR1 antagonist group(AM251),AM251 and HBYYRJ combination group.Each group consists 7 compound holes and was treated for 24 hours,LX-2 cells and culture medium from each experimental group are collected and tested according to the experimental design.MTT assay was adopted to measure the rate of proliferation of LX-2 cells.The expressions of CBR1 and α-SMA were evaluated by Western blot,Enzyme digestion method was used to detect the content of hydroxyproline(Hyp).Results:Compared with the control group,Haobie Yangyin Ruanjian(HBYYRJ)and AM251 could significantly reduce the expressions of CBR1 and α-SMA protein,inhibit the proliferation of LX-2 cells and weaken the secretion of hydroxyproline(Hyp)(P＜0.05).Compared with the AM251 group,the combination of the two drugs,HBYYRJ could enhance the effects of CBR1 antagonist AM251 on the proliferation of LX-2 cells,the expression of CBR1 and α-SMA protein and the secretion of Hyp,and the two drugs had synergistic effect(P＜0.05).Conclusions:Haobie Yangyin Ruanjian can inhibit the activation and proliferation of hepatic stellate cells by interfering with cannabinoid receptor signaling pathway.
万方数据
维普
