Effect of Shexiang Baoxin Pill on regulating PINK1/Parkin signaling pathway in diabetic retinopathy rats
[Objective]To investigate the effect of Shexiang Baoxin Pill(SBP)on mitochondrial autophagy and PTEN-induced putative kinase 1(PINK1)/cytoplasmic E3 ubiquitin-ligase enzyme(Parkin)signaling pathway in diabetic retinopathy(DR)rats.[Methods]DR rats were separated into normal group(control group),model group(DR group),low dose Shexiang Baoxin Pill group(SBP-L group),high dose Shexiang Baoxin Pill group(SBP-H group),and high dose Shexiang Baoxin Pill+rapamycin group(SBP-H+RAP group).The levels of fasting blood sugar(FPG),total cholesterol(TC),triglyceride(TG)and low-density lipoprotein cholesterol(LDL-C)of blood lipids were detected in rats of each group;HE staining was applied to observe diabetic retinopathy of rats in each group;transmission electron microscopy was applied to observe the mitochondrial morphology of rat cells in each group;immunohistochemistry was applied to detect the expression of mitochondrial autophagy related proteins SQSTM1/P62,LC3-Ⅱ;Western blot was applied to detect the expression of PINK1 and Parkin proteins of rats in each group.[Results]Compared with the control group,the retinal structure of the DR group was severely damaged,with disordered cell arrangement,obvious edema,widened cell gaps,obvious mitochondrial damage and swelling,and abnormal morphology,the expression levels of FPG,TC,TG,LDL-C,LC3-Ⅱ,PINK1,and Parkin were obviously increased,the expression level of SQSTM1/P62 was obviously reduced(P＜0.05);compared with the DR group,the structure of retinal cells in the SBP-L and SBP-H groups improved,the cell arrangement gradually became neat,the cell edema decreased,the morphology of cells and mitochondria tended to be normal,and mitochondrial swelling decreased,the expression levels of FPG,TC,TG,LDL-C,LC3-Ⅱ,PINK1,and Parkin were obviously reduced,the expression level of SQSTM1/P62 was obviously increased(P＜0.05);compared with the SBP-H group,the structural damage of retinal cells in SBP-H+RAP group worsened,with disordered cell arrangement,obvious edema,increased mitochondrial swelling,and abnormal morphology,the expression levels of FPG,TC,TG,LDL-C,LC3-Ⅱ,PINK1,and Parkin were obviously increased,the expression level of SQSTM1/P62 was obviously reduced(P＜0.05).[Conclusion]Shexiang Baoxin Pill can inhibit mitochondrial autophagy in diabetic retinopathy rats by inhibiting PINK1/Parkin signaling pathway.
万方数据
维普
