Simultaneous determination of six active ingredient in Qinggan Tongyin by UHPLC-MS/MS and pharmacokinetics in rats
[Objective]The aim of this experiment is to study the pharmacokinetic characteristics of the active ingredients of Qinggan Tongyin in rat plasma.[Methods]An ultra performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS)method was established to detect six active ingredients in rat plasma,including glycyrrhetinci acid,luteolin,apigenin,paeoniflorin,forsythiaside A,and phillyrin and investigate the pharmacokinetic characteristics of six components in rats after oral administration of Qinggan Tongyin.Using WELCN Ultimate XB-C18 column(2.1 mm×100 mm,3 μm)Separation;The mobile phase is 0.1%formic acid water(A)-acetonitrile(B);Gradient elution;Injection volume 3 μL;The flow rate is 0.2 mL/min;column temperature is 40℃;Simultaneous determination of positive and negative ions in multi reaction monitoring mode(MRM).[Results]The research results showed that the linear relationships of various components in rat plasma samples were good(r＞0.99),and its intra-day and inter-day precision,accuracy,stability,matrix effect and extraction recovery rate all met the analysis requirements of biological samples.The pharmacokinetic parameter results show that the t1/2z of each component is 1.18 to 34.12 h,Tmax is 0.44 to 19.33 h,Cmax is 9.00 to 318.09 μg/L,AUC0～t is 12.40 to 15 969.32(h·μg)/L,and MRT0～t is 2.23 to 23.19 h.[Conclusion]The detection method established in this experiment is fast,accurate,and has good repeatability,and can be applied to the pharmacokinetic study of glycyrrhetinci acid,luteolin,apigenin,paeoniflorin,forsythiaside A,and phillyrin in rat plasma.
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