Protective effect and mechanism of Dahuang Huanglian Xiexin Decoction on acute gastric ulcer by inhibiting NLRP3 inflammatome activity
[Objective]Study on the protective effect of Dahuang Huanglian Xiexin Decoction on acute gastric ulcer mice by inhibiting NOD-like receptor thermal protein domain associated protein 3(NLRP3)inflammasome under different immersion conditions and explore its mechanism of action and screen the optimal impregnation conditions.[Methods]Acute gastric ulcer model was established in mice by gavage with anhydrous ethanol,and Dahuang Huanglian Xiexin Decoction was administered under different extraction conditions for the experiment,while MCC950 inhibitor was selected as the positive drug.Hematoxylin-eosin(HE)staining of gastric tissue was performed on each group of mice to observe pathological changes in gastric tissue;Statistical ulcer index(UI);Use a pH test paper to test the potential of hydrogen(pH)value of gastric juice;Detection of gastric juice pepsin activity(PA);Detection of the level of interleukin-1β(IL-1β)and interleukin-18(IL-18)in serum using enzyme-linked immunosorbent assay(ELISA);Western blot was used to detect the protein expression of NLRP3 and Caspase-1 in the gastric tissue of drug treated mice.[Results]Compared with the blank group,the model group mice showed significant ulcerative lesions in the gastric tissue and abnormal indicators.Compared with the model group,the drug group can reduce the ulcer index of ethanol induced acute gastric ulcer model mice(P＜0.05 or P＜0.01),inhibit gastric acid secretion(P＜0.05)and pepsin activity(P＜0.01).By comparing the grayscale values of various drug groups with the grayscale values of control model proteins,it was found that the drug group was able to reduce the expression of NLRP3 and Caspase-1 proteins(P＜0.05 or P＜0.01).Finally,a comprehensive evaluation was conducted on the efficacy of Dahuang Huanglian Xiexin Decoction under different soaking conditions,and the results showed that the E(85℃,15 min)group had the highest score.[Conclusion]Dahuang Huanglian Xiexin Decoction has a protective effect on acute gastric ulcer mice induced by anhydrous ethanol gavage,and its mechanism may be related to the inhibition of NLRP3 inflammasome activity.By inhibiting the expression differences of NLRP3 inflammasomes among different drug groups,the optimal impregnation conditions of 85℃and 15 minutes were obtained.
Dahuang Huanglian Xiexin Decoctionacute gastric ulcerimmersion methodNLRP3 inflammatory body
国家科技期刊平台
NSTL
万方数据
维普
