Study on the effect of polygonatum polysaccharides on the TGF-β/Smad signal pathway in placental tissue of pregnancy induced hypertension rats
[Objective]To investigate the impacts and mechanism of polygonatum polysaccharide on placental function in pregnancy induced hypertension model rats.[Methods]Pregnant female rats were randomly grouped into control group,model group,polygonatum polysaccharide(low,medium and high)dose groups(100,200,400 mg/kg)and magnesium sulfate group(100 mg/kg),with 10 rats in each group.In addition to the control group,rats in other groups were subcutaneously injected with L-arginine methyl ester to build the hypertensive model of pregnancy.Blood pressure,24-hour proteinuria,fetal rat body mass and placenta weight were measured;hematoxylin-eosin(HE)was applied to detect the histopathology of placenta;the contents of interleukin(IL)-6,IL-10 and tumor necrosis factor-α(TNF-α)in placenta were detected by enzyme linked immunosorbent assay(ELISA);Western Blot was applied to detect the expression of tight junction protein-1(ZO-1),blocking protein(OCLN),vascular endothelial growth factor(VEGF),transforming growth factor-β1(TGF-β1),p-Smad3 and Smad3 proteins in placenta.[Results]Compared with the control group,the blood pressure,24-hour urine protein,IL-6,TNF-α contents and TGF-β1,p-Smad3 protein expression in the model group were significantly higher;the body weight,placenta weight,IL-10 content and the expression of ZO-1,OCLN and VEGF protein of fetal rats were significantly lower(P＜0.05),and pathological damage occurred in placenta tissue.Compared with the model group,the blood pressure,24-hour urine protein,IL-6,TNF-α contents and TGF-[31,p-Smad3 protein expression of rats in administration groups were significantly lower,the body weight,placenta weight,IL-10 content and the expression of ZO-1,OCLN and VEGF protein of fetal rats were significantly higher(P＜0.05),and the pathological damage of placenta was alleviated.[Conclusion]Polygonatum polysaccharide can inhibit the activation of TGF-β/Smad signal pathway in the placenta of pregnancy induced hypertension model rats and improve the placental dysfunction.
pregnancy induced hypertensionplacental functionpolygonatum polysaccharideTGF-β/Smad signal pathway
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