Inhibitory effect and mechanism of Xiaoyan Decoction combined with Apatinib Mesylate on transplanted tumor of gastric cancer bearing mice
[Objective]To investigate the antitumor effect and antitumor mechanism of Xiaoyan Decoction combined with Apatinib Mesylate on transplanted tumor of gastric cancer bearing mice.[Methods]Gastric cancer MFC cells were inoculated subcutaneously into BALB/C male mice.After tumor formation,they were randomly divided into model group,Xiaoyan Decoction group,Apatinib group and combined drug group.The non model group was blank control group,with 5 rats in each group.Mice in each group were given normal saline and corresponding drugs by gavage for 14 days.The living conditions and tumor volume changes of mice were observed and recorded.After administration,mice in each group were killed,tumor tissues were taken,weighed and recorded,and the expression of PI3-K/Akt signal pathway and angiogenesis related protein in tumor tissues were detected by Western blot.[Results]The observation of the living conditions of mice in each group showed that the living conditions of mice in Xiaoyan Decoction group and Apatinib group were better than those in model group,and the improvement of mice in combined drug group was more obvious;compared with the tumor bearing model group,the tumor volume and mass of Xiaoyan Decoction group,Apatinib group and combined drug group were significantly reduced,especially in the combined drug group;compared with the tumor bearing model group,the protein expressions of p-PI3K,p-AKT,Bcl-2,MMP-9,MMP-2,VEGFA and VEGFR-2 in Xiaoyan Decoction group,Apatinib group and combined drug group were significantly down regulated,and the protein expressions of Cleaved Caspase-9 and Bax were significantly up-regulated(P＜0.05).Compared with the single drug group,the combined drug group had more obvious regulatory effect on PI3K/Akt signal pathway and angiogenesis related protein expression.[Conclusion]Xiaoyan Decoction combined with Apatinib can effectively inhibit the growth of transplanted tumor in gastric cancer mice and improve the living conditions of mice by regulating the expression of PI3K/Akt signal pathway and angiogenesis related protein.
Xiaoyan DecoctionApatinibgastric cancerPI3K/AKT signal pathwayangiogenesis
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维普
