Effects of Qidi Tongbian Prescription on mice with slow transit constipation based on Wnt/β-catenin pathway
[Objective]To study the specific regulatory effects of Qidi Tongbian Prescription on slow transit constipation(STC)mice based on Wnt/β-catenin signaling pathway.[Methods]The 30 BALB/c mice were randomly divided into 6 groups,five mice in each group.Except the blank group,the STC mouse model was established by intragastric administration of loperamide hydrochloride(10 mg/kg)for 14 days,followed by intragastric intervention of high,medium and low doses(18.906,9.453,4.727 g/kg)of Qidi Tongbian Prescription and positive drug lactulose(3 g/kg)for 14 days.Fecal moisture content,fecal quantity and small intestine advancement rate were measured.Colon histological changes were evaluated by HE staining.Gene and protein expression levels of Wnt-3a and β-catenin were detected by PCR and immunohistochemistry.And the expression level of Ano1 protein in Cajal interstitial cells(ICC)was detected by immunofluorescence staining.The expression levels of Occludin,5-hydroxytryptamine(5-HT)and aquaporin 3(AQP3)were detected by ELISA.[Results]Compared with blank group,the small intestinal propulsion rate,fecal quantity and water content in model group were significantly decreased(P＜0.01).The intervention of Qidi Tongbian Prescription could effectively improve the small intestinal propulsion rate,fecal quantity and water content(P＜0.05),and improve the histological lesions.Compared with blank group,the expression of Wnt-3a,β-catenin mRNA(P＜0.05)and protein(P＜0.01)increased in model group,while the expression of Ano1 protein decreased(P＜0.01),Occludin protein decreased(P＜0.01).The protein expressions of AQP3(P＜0.01)were increased.The prognosis showed that Wnt-3a protein,β-catenin mRNA and protein expression decreased(P＜0.05),Ano1 protein expression increased(P＜0.01),Occludin protein expression increased(P＜0.01).The protein expressions of AQP3 were decreased(P＜0.05).[Conclusion]Qidi Tongbian Prescription may play a role in the treatment of STC by inhibiting the overactivation of Wnt/β-catenin signaling pathway.
万方数据
维普
