[Objective]To establish and methodologically validate an HPLC-UV method for the determination of ginkgolides A,B,and C.The method was validated by the HPLC-UV method.[Methods]The investigation included detection wavelength,eight different mobile phase systems and their ratios,injection volume,flow rate and column temperature.The method validation examined the specificity,precision,accuracy,linear range,repeatability,system suitability,spiked recovery and stability.[Results]The separation was carried out on an Agilent TC-5 C18 column at a detection wavelength of 220 nm with a mobile phase of water-methanol(70∶30)at an injection volume of 20 μL,a column temperature of 30℃,and a flow rate of 1.0 mL/min.The linearity of the method was good,and the peaks of the chromatograms did not interfere with each other.[Conclusion]The method is accurate,effective,economical,easy to obtain and simple to operate,and can provide a reference for the determination of other ginkgolides.
万方数据
维普
