[Objective] To study the growth conditions of Crifola frondosa on 9 different kinds of mother culture media and effect of different carbon and nitrogen sources on mycelium growth. [Methods] The same mycelia block was put into tubes and plates in each group to observe and record and shoot growth conditions of Crifola frondosa. [Results] In the formula B, namely, integrated PDA medium, had the shortest full pipe time (18 d), the growth vigour (mycelium white, branch number, colony integrity, thick) was better than any others; fructose was the best carbon source in 5 tested carbon sources and beef extract was the best nitrogen source in 7 tested nitrogen sources. Organic nitrogen was more suitable growth of Crifola frondosa than inorganic nitrogen sources. [Conclusion] Integrated PDA medium is the best formula of mother culture medium for Crifola frondosa; fructose and beef extract are the best carbon and nitrogen sources.