Determination of 6-gingerol in ginger by high performance liquid chromatography
[Objective] To establish a HPLC method for the determination of 6-gingerol in ginger. [Methods] Ginger was extracted by heat reflux with 80% methanol for 2 hours, the drug ratio was 1 :100, and the Hypersil C18 ODS (250 mm × 4.6 mm, 5 μm) column was used to determine. The mobile phase consisted of methanol-water-isopropyl-alcohol-glacial acetic acid (3836:12:4); The flow rate was 0.8 mL/min. The detection wavelength was 280 nm. The column temperature was kept at 34℃. [Results] The linear range was between 0.021 6～0.432 0 μg. And the average recovery of the method was (100.79±2.84) % and the RSD was 2.84%. [Conclusion] The results show that this assay method is simple and accurate. And the method can be used to determine the contents of 6-JF in Zingiber of-ficinale Rose.
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NSTL
万方数据
维普
