Isolation and structural identification of constituents from Dioscoreae spongiosae Rhizoma
Objective] Study on chemical constituents of Dioscoreae spongiosae Rhizoma. [Methods] The chemical constituents were obtained by ODS, Sephadex LH-20 and PHPLC chromatorgraphy isolation, and identified by chemical and spectral analysis. [Results] The structures of seven comounds were elucidated to be tachioside (1), isotachioside (2), koaburaside (3), 2,6-dimethoxy-4-hydroxyphenol-1-O-β-D-glucopyranoside (4), icariside D2 ( 5 ) , benzyl alcohol O-β-D-glucopyranoside ( 6 ) , and adenosine ( 7 ) , respectively . [ Conclusion ] Compounds 1~3 were obtained from Dioscoreae genus firstly, and 4~7 was isolated from the plant for the first time.