Analysis on the determination and quantity transfer of multi index components in Qianghuo Shengshi Decoction
[Objective]Establish the High Performance Liquid Chromatography(HPLC)content determination method of multi-index components in Qianghuo Shengshi Decoction(QSD)substance benchmarks,to analysis the quantity transfer of multi-index components.[Methods]Preparing 15 batches of substance benchmarks freeze-drying powder(lyophilized powder)samples of QSD.The specific components,including Notopterol,Columbianadin,5-O-methylvisammioside,Vitexicarpin,Isoimperatorin,and Ammonium Glycyrrhizinate were used as indexes to establish the HPLC method for quantitative evaluation,and define the contents and the transfer rate range of multi-index components and the paste-forming rate range to analysis on the quantity transfer of multi index components in decoction pieces to substance benchmarks.[Results]The contents and transfer rates of the 15 batches of substance benchmarks were 1.09～1.70 mg/g and 10.17%～32.62%of Notopterol;Columbianadin were 0.06～0.20 mg/g and 7.30%～10.85%;5-O-methylvisammioside were 1.27～2.81 mg/g and 66.13%～92.13%;Vitexicarpin were 0.09～0.59 mg/g and 5.24%～13.37%;Isoimperatorin were 0.03～0.28 mg/g and 0.94%～1.92%;Ammonium Glycyrrhizinate were 12.74～33.78 mg/g and 48.96%～62.56%,respectively.The paste-forming rate ranged from 30.09%to 34.35%.[Conclusion]The content determination method of standard multi-index components of classical famous prescription QSD was preliminarily established by HPLC,which provided a basis for the quality control of the follow-up research and the development of related preparations of QSD.
classical prescriptionQianghuo Shengshi Decoctionsubstance benchmarkquantity transfer
万方数据
维普
