Mingjing Granules combined with leizumab on vascular membrane fibrosis associated proteins in wet age-related macular degeneration
[Objective]To observe the effects of Mingjing Granules(MJKL)combined with lezumab intravitreal injection on the expression of vascular membrane and fibrosation-related proteins in experimental wet age-related macular degeneration(nAMD),explain the mechanism of action of MJKL in the treatment of nAMD,and provide theoretical basis for the clinical treatment of traditional Chinese medicine(TCM)combined with anti-vascular endothelial growth factor(VEGF)drugs.[Methods]Experimental nAMD fibrovascular membrane model was established by two-stage laser photocoagulation.BN rats were randomly divided into three groups:model group,anti-VEGF group,MJKL +anti-VEGF group.Model group:distilled water was given intragastric administration;Anti-VEGF group:intravitreous cavity was injected with leizumab injection;MJKL+ anti-VEGF group:Leizumab injection was injected into the vitreous cavity,and MJKL was given intragastric administration.Ten BN rats were not modeled and were fed as normal control group.After 40 days of model making,fundus lesion morphology,lesion exudation area and MD value were observed by fundus photography(FP),fundus angiography(FFA),optical coherence tomography(OCT),retinal pigment epithelium(RPE)-choroid-sclera film.The changes of retinal structure were observed by histopathology,the expression and distribution of collagenia-1,Fibronectin,α-SMA,TGF-β1 were detected by immunofluorescence,and collagenia-1 mRNA and Fibronectin mRNA were detected by qRT-PCR.The relative expression of α-SMA mRNA and TGF-β1 mRNA.[Results]The fibrovascular membrane model was established 40 d after two-stage laser modeling.The lesion lesion area,optical density(MD)value and lesion height in anti-VEGF group were significantly decreased compared with model group(P＜0.05),and lesion area and retinal structural damage degree were significantly decreased.Compared with the anti-VEGF group,MJKL+ anti-VEGF group significantly reduced the MD value and lesion height in lesion exudation(P＜0.05),and the lesion area and retinal structural damage degree were also significantly reduced.The fluorescence intensity of Collagen 1,Fibronectin,α-SMA and TGF-β1 in the model group was significantly higher than that in the normal group(P＜0.05),while the fluorescence intensity in the anti-VEGF group was significantly lower than that in the model group(P＜0.05).The fluorescence intensity of Collagen 1,Fibronectin and TGF-β1 in MJKL+ anti-VEGF group was significantly lower than that in anti-VEGF group(P＜0.05).The relative expression levels of Collagen 1 mRNA,Fibronectin mRNA,α-SMA mRNA and TGF-β1 mRNA in the model group were significantly higher than those in the normal group(P＜0.05),while those in the anti-VEGF group were significantly lower than those in the model group(P＜0.05).MJKL+ anti-VEGF group was significantly lower than anti-VEGF group(P＜0.05).[Conclusion]MJKL combined with anti-VEGF drugs can reduce the expression of Collagen 1,Fibronectin,α-SMA,TGF-β1 proteins,which can better inhibit the growth of experimental nAMD fiber vascular membrane than the single application of anti-VEGF drugs.
wet age-related macular degenerationMingjing GranulesTwo-stage laser inductionfibrovascular membraneTGF-β1fibrosis-associated protein
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维普
