Study on pharmacokinetic interaction by the combination of Xuebijing Injection and Azithromycin in acute pneumonia mouse and inflammation cells
[Objective]To determine the pharmacokinetic properties of combination of Xuebijing Injection and Azithromycin,Hydroxy safflower yellow A and Paeoniflorin by liquid chromatography-mass spectrometry/mass spectrometry(LC-MS/MS)on acute pneumonia animal and cells level.[Methods]Acute pneumonia mouse and inflammation cells model were established by lipopolysaccharides(LPS).The chromatographic separation was achieved on a Waters ACQUITY UPLC BEH C18(100 mm×2.1 mm,1.7 μm)column;The mobile phase was consisted of 0.1%formic acid water-formic acid acetonitrile at a flow rate of 0.3 mL/min with gradient elution,the mass spectrums were scanned by Essential Science Indicators(ESI)+and ESI-multiple reaction monitoring mode.[Results]With respect to the overall pharmacokinetics in model animals,the Area Under the Curve(AUC)of Azithromycin,Hydroxy safflower yellow A and Paeoniflorin were significantly increased(P<0.05),Cmax and Tmax of Azithromycin,Hydroxy safflower yellow A were significantly increased(P<0.05)and the AUC of lung tissue also increased(P<0.01).Based on cell level pharmacokinetics,the AUC of Azithromycin in RAW264.7 cells increased significantly(P<0.01),while the AUC of Hydroxy safflower yellow A and Paeoniflorin in cells did not change significantly(P>0.05).[Conclusion]There was a pharmacokinetic interaction after the combination of azithromycin and Xuebijing,and the pharmacokinetic characteristics of the cells were significantly changed.
Xuebijing InjectionAzithromycincombination of Chinese and Western medicinepharmacokinetic interaction
国家科技期刊平台
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