The effect of cirsimaritin on rheumatoid arthritis in mice based on STING/NF-κB signaling pathway
[Objective]To evaluate the effect of thistle flavescens on collagen-induced arthritis(CIA)in mice by regulating STING/NF-κB signaling pathway.[Methods]Forty SPF grade female C57BL/6 mice were divided into blank control group(Sham),model group(CIA),methotrexate group(MTX,1 mg/kg)and cirsimaritin group(JHS,100 mg/kg).The mice rheumatoid arthritis models were established by collagen immunoassay.After 4 weeks of drug intervention,the experiment was completed.Swelling degree of the hind PAWS,levels of TNF-α,IL-1β,IL-6,IL-10,MDA and SOD activity in serum were detected,morphological changes of the left knee joint were observed,and the expression level of STING/NF-κB pathway key proteins in the right knee joint was measured.[Results]Compared with CIA group,the swelling degree of hind PAWS in MTX group and JHS group was significantly decreased(P＜0.01),the levels of TNF-α,IL-1[3,IL-6,IL-10 and MDA in serum were significantly decreased,while the activity of SOD was significantly increased(P＜0.01),the inflammatory cell infiltration of knee joint was significantly decreased,and the cartilage tissue damage was significantly alleviated.The expression levels of STING and P-NF-κB p65/NF-κB protein in knee joint were significantly decreased(P＜0.01).[Conclusion]Cirsimaritin can significantly improve rheumatoid arthritis,and its mechanism may be related to reducing inflammation and oxidative stress damage by regulating STING/NF-κB signaling pathway.
万方数据
维普
