Exploring the effects of compound Juema Capsules on microcirculation in hemorrhagic shock rats with low temperature and hypoxia based on orthogonal design
[Objective]Based on orthogonal design,to investigate the effects of different conditions(air oxygen content,ambient temperature and drug concentration)on microcirculation in rats with hemorrhagic shock(HS)and to provide an optimal solution for the prevention of plateau hemorrhagic shock.[Methods]In this study,three factors,altitude(simulated with air oxygen content)[3 551.7 m(13.5％),3 080.7 m(14.5％),2 751 m(15.2％)],ambient temperature(5-10 ℃,10-15 ℃,15-20 ℃)and drug concentration[low(27 mg/kg),medium(54 mg/kg)and high(81 mg/kg)dose of compound Juema Capsule]were selected,and three levels of each factor were chosen,and the experiment was carried out by using a three-factor,three-level orthogonal design in accordance with the orthogonal table of L9(34)to obtain a total of nine Combinations.Twenty-seven 350-400 g male SD rats were divided into nine groups according to the orthogonal design of the intervention scheme,and each group was repeated three times for the modeling of HS rats.Mean arterial pressure(MAP),heart rate(HR),and mesenteric microcirculation indexes[including vascular flow velocity,perfusion vascular density(PVD)and microcirculation flow index(MFI)]were collected and recorded at seven time points during the experiment:before bloodletting(T),before resuscitation(T0),1 h after resuscitation(T1),2 h after resuscitation(T2),3 h after resuscitation(T3),4 h after resuscitation(T4),and 5 h after resuscitation(T5).Relevant statistical methods were used to analyze the results,first to screen the optimal observation time point of the experiment,and then to select the optimal solution for improving the plateau HS rats under the present experimental conditions.[Results]Screening for the best observation time point of the experiment:seven time points of MAP,HR,and mesenteric flow rate were compared among nine groups of rats.1)MAP:①The trend of MAP chang es in each group of rats at different time points is consistent(F=1.471,P=0.051);②There was no statistically significant difference between groups(F=1.002,P=0.467);③The MAP values of each group were not all equal at different time points(F=417.804,P＜0.001).2)HR:①The HR of each group of rats showed consistent trends at different time points(F=0.782,P=0.730);There was no statistically significant difference between groups(F=0.647,P=0.729);③The HR values of each group were not all equal at different time points(F=947.477,P＜0.001).3)Mesenteric flow velocity:①The trend of changes in mesenteric flow velocity among different groups of rats at different time points is different(F=4.933,P＜0.001).②There was no statistically significant difference in inter group flow velocity(F=2.141,P=0.086);③At different time points,each group of T was not completely equal(F=82.425,P＜0.001).4)Summary:Based on the data,T1 was selected as the best experimental observation point.Selection of the optimal solution for the conditions of this experiment:①The best combination for improving blood vessel flow rate is the second group;②The best combination for improving PVD is the second group.③The best combination to improve MFI is the second group.④Summary:The second group(i.e.oxygen content of 13.5％,temperature of 10-15 ℃,and drug concentration of medium dose)had the best combination within the experimental conditions.[Conclusion]Among the five observation points after resuscitation of HS rats,T1 is the best observation point in the experiment;the medium dose of compound Juema capsule is the best resuscitation effect for HS rats around 3 500 m.
万方数据
维普
