Analysis of monosaccharide composition and molecular mass of Scrophularia ningpoensis polysaccharides from different regions
[Objective]Determination the monosaccharide composition of 10 batches of polysaccharides in Scrophularia ningpoensis from different regions,and determination the relative molecular mass and content of Scrophularia ningpoensis polysaccharides.[Methods]The monosaccharide composition of 10 batches of Scrophularia ningpoensis polysaccharides from different regions were determined by high performance liquid chromatography(HPLC)for pre-column derivatization of 1-phenyl-3-methyl-5-pyrazolone(PMP).The mixed monosaccharide reference substance based on the monosaccharide composition of Scrophularia ningpoensis polysaccharides was prepared,and the content of Scrophularia ningpoensis polysaccharides were determined by phenol-sulfuric acid.The relative molecular mass of Scrophularia ningpoensis polysaccharides was determined by the high performance gel permeation chromatography(HPGPC).[Results]Scrophularia ningpoensis polysaccharides were composed of mannose,rhamnose,galacturonic acid,glucose,galactose,and arabinose,with the highest content being galacturonic acid,followed by galactose.The six monosaccharide components showed good linear relationships within their respective linear ranges,with sample recovery rates ranging from 97.71%to 103.89%.The polysaccharide molecular mass of 10 batches of Scrophularia ningpoensis polysaccharides ranges from 89 536 to 122 744 Da,and the polysaccharide content ranges from 70.09%to 83.70%.[Conclusion]There are significant differences in the monosaccharide composition,molecular mass and content of Scrophularia ningpoensis polysaccharides from different regions.This paper can provide a basis for further research on Scrophularia ningpoensis polysaccharides.
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