To study the mechanism of"warming effect"of fire needling therapy in improving the inflammatory microenvironment in the acute stage of spinal cord injury
[Objective]To explore the mechanism of fire needling therapy in improving the inflammatory microenvironment of acute spinal cord injury.[Methods]24 female SD rats were randomly divided into blank group,sham operation group,model group and fire needling group,with 6 rats in each group.The SCI model was established by modified Allen's method.In the sham-operation group,T9 and T10 spinous processes and partial laminae were removed without intervention.The model group was not intervened after modeling.After modeling,the rats in the fire needling group were stimulated with fire needling at T7,T8,T11,T12 0.5cm below the spinous process and at the left and right lateral points,a total of 12 acupoints.Infrared imaging technology was used to measure the temperature changes of rats before intervention,immediately after intervention,10,20 and 30 min after intervention.The protein expression levels of Toll-like receptors 4(TLR4),myeloid differentiation primary factor88(MyD88)and nuclear factor-kappa B(NF-κB)in spinal cord tissues were detected by Western blot.The levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-4(IL-4),interleukin-6(IL-6)and interleukin-10(IL-10)in serum were detected by enzyme-linked immunosorbent assay.[Results]1)Infrared imaging results showed that the temperature of the fire needling group increased significantly immediately after intervention,decreased 10min after treatment,and then gradually stabilized.2)Western blot results showed that compared with the model group,the relative expression of TLR4 in the spinal cord tissue of the fire needling group was decreased(P<0.05),and the relative expressions of MyD88 and p-Nf-κb were decreased,but the differences were not statistically significant(P>0.05).3)Elisa results:Compared with the model group,the content of serum TNF-α in the fire needling group was decreased(P<0.05).Compared with the model group,the serum levels of IL-1β and IL-6 in the fire needling group were decreased,and the difference was not statistically significant(P>0.05).Compared with the model group,the serum levels of IL-4 and IL10 in the fire needling group were increased(P<0.05).[Conclusion]The"warming effect"of fire needling therapy can regulate the pro-inflammatory/anti-inflammatory balance through TLR4/MyD88/NF-κB pathway to improve the inflammatory microenvironment in the acute stage of spinal cord injury.
fire needlewarming effectspinal cord injuryinflammatory microenvironmentacupuncture needle
国家科技期刊平台
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