Soil samples were collected from the lowland rainforest secondary forests,montane rainforest secondary forests,and montane rainforest primary forests in the Jianfengling of Hainan Island,absolute quantification PCR and high-throughput sequencing technology were used to explore the diversity and composition of carbon sequestering microbial community structure in soil and its influencing factors.The results showed that both α diversity and gene quantity of carbon-fixing microorganisms were higher in lowland rainforest secondary forest than in montane rainforest primary forest.The dominant phyla of soil carbon-fixing microorganisms in the Jianfengling rainforest were Actinomycetota(11.95％-33.73％)and Pseudomonadota(44.28％-70.60％),the dominant genera were Bradyrhizobium(11.53％-31.06％),Nocardia(4.97％-17.07％),Mesorhizobium(4.44％-12.97％),Mycobacterium(2.62％-9.20％),Blastochloris(4.34％-10.12％),Aromatoleum(1.97％-5.39％),Mycolicibacterium(1.71％-3.78％),Azospirillum(1.97％-2.65％)and Methylibium(1.22％-3.77％),and facultative autotrophs were predominant.PCoA and LEfSe analyses revealed the differences in soil carbon sequestration microbial communities among the three forest types of tropical rainforest,with marker species belonging to Actinomycetes,γ-Proteobacteria,α-Proteobacteria andβ-Proteobacteria,respectively.RDA analysis showed that total nitrogen,total phosphorus,organic carbon and readily oxidizable carbon were important soil factors for the differences.
维普
万方数据
