Relying on the long-term positioning experiment of rice soil ex-situ soil of the Taoyuan Agricultural Ecology Experimental Station of the Chinese Academy of Sciences,three paddy soils derived from different parent materials were selected from Yingli in Guangdong Province(YL,basalt weathering material),Yingtan in Jiangxi Province(YT,quaternary red clay material)and Taoyuan in Hunan Province(TYG,river alluvium material),physicochemical properties and nitrification rates were measured using conventional agrochemical soil analysis methods,and the similarities and differences in ammonia-oxidizing archaea(AOA)and ammonia-oxidizing bacteria(AOB)gene abundances and community structure were analyzed using real-time quantitative PCR and Illumina MiSeq sequencing.The results showed nitrification rate was presented as YT>YL>TYG except at tillering stage.And the abundance of AOA dominated in YL and YT,with AOA/AOB ratios between 0.72 and 3.05 and 0.98 and 1.52,respectively.In TYG,the abundance of AOB was dominant,with an AOA/AOB ratios between 0.21 and 0.75.Parent material has a significant influence on AOA and AOB community structure,the predominant genera were different in AOA while Nitrosospira dominant in AOB with three soils.The redundancy analysis(RDA)showed that pH and DOC were the most important factors influencing AOA and AOB community structure.In conclusion,there are differences in the physicochemical properties in paddy soils derived from different parent materials in the same environment,which affects the abundance and community structure of AOA and AOB.
维普
万方数据