Effects of Combined Applications of Potassium Humate with Different Microbial Agents on Soil Improvement of Mine Wasteland
In order to study the effects of Trichoderma harzia(TH),Bacillus glia(BM)and Streptomyces flavus(SM)mixed with potassium humate(HAK)on soil improvement,9 treatments including 1)HAK,2)TH+HAK,3)BM+HAK,4)SM+HAK,6)TH+BM+HAK,7)TH+SM+HAK,8)BM+SM+HAK,and 9)TH+BM+SM+HAK were set up for a pot experiment.Soil physicochemical and enzyme activities were measured,and soil improvement effects were ranked by grey correlation degree theory.The results showed that single HAK significantly improved soil moisture and phosphomonesterase activity,but its effects were lower than other treatments(different bactericide+HAK).In single bacteria+HAK,BM+HAK increased the contents of total nitrogen and available phosphorus and the activities of soil urease compared with the blank control,and increased the content of soil alkali-hydrolytic nitrogen and organic matter compared with single HAK,TH+HAK increased the activities of soil sucrase and phosphomonesterase compared with single HAK,and SM+HAK significantly decreased the activity of urease compared with the blank control.Compared with BM+HAK,TH+BM+HAK significantly increased the contents of alkali-hydrolytic nitrogen and available phosphorus,but its effects in increasing soil enzyme activities were between those of TH+HAK and BM+HAK.The ability of three bacteria+HAK in improving soil was between single and double bacteria+HAK.According to the results of grey correlation degree analysis,the weighted correlation degree of BM+HAK was the highest(0.972).In conclusion,BM+HAK has the best improvement effect and can be used as the optimal combination for soil quality improvement.
Abandoned mine soilPotassium humateMicrobial agentSoil physical and chemical propertiesSoil enzyme activity
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