Gene Abundance of N2O Reducers in Rhizosphere Soils of Typical Chinese Fir Plantations from Fujian Province
In order to reveal the abundance of N2O reducers and their critical driving factors in the rhizosphere soils of typical Chinese fir plantation from Fujian Province,rhizosphere soil samples under Chinese fir plantation from three typical forest farms in Fujian Province were collected.The abundance of functional genes of soil N2O reducers,namely nosZⅠ and nosZⅡ,were determined using real-time quantitative PCR.Results showed that the abundance of nosZⅠ gene was significantly higher than that of nosZⅡ in the rhizosphere soils of Chinese fir plantation in all the three typical forest farms,which suggested that nosZⅠ-type N2O reducers dominate in the acidic rhizosphere soils of Chinese fir plantation,possibly due to the fact that the nosZⅡ-type N2O reducers are more sensitive to low pH conditions.Among the three forest farms,the abundance of nosZⅠ gene was 2.53 × 107 copies/g in Wuyishan National Park,and significantly higher than 9.27 × 106 copies/g in Sanming Forest Ecosystem National Observation and Research Station and 8.90 × 106 copies/g in Shanghang Baisha Stated-owned Forest Farm.However,there was not significant difference in nosZⅡ abundance among the three forest farms.Random forest analysis showed that soil pH was the key environmental factor regulating the abundance of nosZⅠ and nosZⅡ.In conclusion,the abundance of nosZⅠ gene varies substantially in the rhizosphere soils of Chinese fir plantation from three typical forest farms in different regions of Fujian Province,while soil pH is the most important driving factor.
万方数据
维普
