Study on Sulfated Modification and Anti-tumor Activity of Laminarin
This study was aimed to sulfate modify laminarin and to investigate the relationship of structure and anti-tumor activity.Chlorosulfonic acid-pyridine method was applied for sulfated modification of laminarin.IR and NMR spectra of laminarin and laminarin sulphate (LAMS) were investigated,and the surface structure of laminarin and JAMS were observed with scanning electron microscope.The anti-tumor activities were evaluated by MTT method.The results showed that both laminarin and LAMS showed characteristic absorption peaks of polysaccharide in IR spectra,and LAMS had characteristic absorption peaks of sulfate; the NMR spectra showed that laminarin and LAMS both had β-(1→3) glycoside bond as main chain,and the sulfate group in LAMS was attached to C2-OH and C6-OH.Under scanning electron microscope,there were obvious differences in surface structure between laminarin and LAMS.Laminarin was shown like cloud or sponginess,while LAMS was shown like flakiness or block.MTT results showed both laminarin and LAMS had inhibitory effects on LOVO growth,and the anti-tumor activity of LAMS was higher.In conclusion,sulfated modification changed chemical structure of laminarin and enhanced its anti-tumor activity markedly.
国家科技期刊平台
NSTL
万方数据
维普
