Simultaneous Determination of Chlorogenic Acid, Luteolin, Apigenin and Acacetin in Medicinal Chrysanthemum morifolium by HPLC
A sensitive and specific high performance liquid chromatography (HPLC) method was developed to determine chlorogenic acid,luteolin,apigenin and acacetin in medicinal Chrysanthemum morfolium.The C.morfolium sample was extracted by ionic liquid based dispersive liquid phase microextraction.The chromatographic separation was carded out on a Phenomenex C1s column (250 mm ×4.6 rmm,5 μ.m) with methanol-0.2％ acetic acid (65∶ 35,v∶ v) as mobile phases at a flow rate of 0.8 mL/min.The UV detection wavdength was set at 350 nm,and the column temperature was set at 35 ℃.The linear ranges were 0.0005 ～ 100 μg/mL for chlorogenic acid (r =0.9999),0.00095 ～ 190 μg/mL for luteolin (r =0.9999),0.0008 ～ 160 μg/mL for apigenin (r =0.9997) and 0.00061 ～ 122 μg/mL for acacetin (r =0.9996),respectively;The recoveries of chlorogenic acid,luteolin,apigenin and acacetin were 90.52％ ～ 100.44％,90.88％ ～ 98.58％,94.40％ ～ 98.82％,93.68％ ～ 100.54％,respectively.The developed HPLC method was quick,simple and reproducible for the simultaneous determination of chlorogenic acid,luteolin,apigenin and acacetin in medici hal Chrysanthemum morifolium.
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