Optimization of Extraction Conditions of Garlic Flavonoids Using Response Surface Methodology and Their Antioxidant Property Investigation
The effect of ethanol concentration,material-to-solvent ratio,ultrasonic time and ultrasonic temperature on the extraction yield of total garlic flavonoids was investigated by single factor test in this study.The ultrasonic-assisted extraction conditions were further optimized by the response surface analysis and were determined to be ethanol concentration of 80％,material-to-solvent ratio of 1∶ 16,ultrasonic time of 18 min and ultrasonic temperature of 53 ℃.Under the optimal extraction conditions,the yield of total garlic flavonoids reached 3.99％.In addition,the pyrogallol autoxidation test indicated that the inhibition rate of superoxide anion radical (O2·) of the garlic extract was 60.43％.The result proved that garlic total flavonoids had relative strong antioxidant activity.
国家科技期刊平台
NSTL
万方数据
维普
