In this study, the content of main effective ingredients including baicalin, baicalein and wogonin of wild Scutellaria baicalensis from 10 areas of Shanxi were investigated by HPLC.The results showed that:there was a significant difference in the content of baicalin, baicalein and wogonin in 10 samples of Scutellaria.The content of baicalin, baicalein and wogonin were 5.79 %-15.31%, 0.49 %-2.28 % and 0.07 %-0.42 %, respectively.The content of baicalin had positive correlation with the cultivation latitude of S.baicalensis, the content of baicalin in S.baicalensis samples from Goubei country, Youyu county and Yinliangshan, Shanyin county was not match up with the standard (8.0%) required by Chinese Pharmacopoeia,while the content of baicalin from Wayaoshang country, Linchuan county was determined to be 15.31%.The content of baicalein and wogonin from Suangjiazhai country,Jiaocheng county was determined to be 2.28% and 0.42%.