首页|模拟加速试验研究陈皮挥发性成分与黄酮类成分动态变化规律

模拟加速试验研究陈皮挥发性成分与黄酮类成分动态变化规律

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采用色谱分析方法,研究陈皮在模拟加速试验条件下挥发性成分和黄酮类成分含量的动态变化规律,以探究陈皮“陈久者良”科学内涵.采用静态顶空进样提取挥发性成分,气相色谱-质谱联用(GC-MS)和自动质谱退卷积定性系统(AMDIS)结合Kováts保留指数(Retention index,RI)对挥发性成分进行分析;利用HPLC-DAD法、UV紫外分光光度法测定样品中的黄酮含量.结果表明陈皮挥发性成分的含量均有下降,低沸点成分下降明显;黄酮类成分总黄酮与橙皮苷的含量呈先增后减的趋势,川陈皮素含量呈增加趋势.模拟加速试验方法用于研究中药贮藏过程中化学成分动态变化结果满意,但指标性成分含量变化对于表征陈皮药效组分整体特性变化具有局限性,应研究建立陈皮在贮藏中主要药效物质的化学特性表征谱,从物质基础变化角度阐释陈皮“陈久者良”科学内涵.
Dynamic Variations of Volatiles and Flavonoids in Citrus Peel during Storage by Simulated Accelerated Test
Through the chromatographic analysis method,researches were carried out on the dynamic variation rules of volatiles and flavonoids in Citrus Peel under the condition of simulated accelerated test,in order to explore the scientific connotation of the concept of "the older one is the better one" for Citri Reticulatae Pericarpium (CRP).With GC-MS and AMDIS combined with Kovats retention index (RI),an analysis was made for volatile components extracted using static headspace;flavonoid content in samples was measured through HPLC-DAD and UV spectrophotometry method.The result showed the contents of volatile components were all decreased and those with low boiling points had a more significant declination;the flavonoids component content of total flavonoids and hesperidin tended to increase at first and then decrease,while the content of nobiletin had an increasing tendency.When simulated accelerated test method was used to study the dynamic variation of the chemical components of traditional Chinese medicine in storage,the result was satisfactory.But there was limitation for the index component content variation to represent the characteristic variation of overall efficacy composition in CRP.A characteristic spectrum of chemical properties should be researched and established for the main efficient materials of CRP in storage,so as to explain the scientific connotation of the concept of "the older one is the better one" for CRP at the point of material basic variation.

Citri reticulatae pericarpiumthe older, the bettervolatile componentsflavonoidssimulated accelerated test

张鑫、刘素娟、王智磊、王福、陈鸿平、刘友平

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成都中医药大学药学院中药材质量标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137

陈皮 陈久者良 挥发性成分 黄酮 模拟加速试验

国家自然科学基金国家基础科学人才培养基金A

81072991J13100342015JY0012

2016

天然产物研究与开发
中国科学院成都文献情报中心

天然产物研究与开发

CSTPCDCSCD北大核心
影响因子:0.783
ISSN:1001-6880
年,卷(期):2016.28(11)
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