Study on UPLC fingerprints and quantitative analysis of multi-components by single marker of Schisandrae Sphenantherae Fructus
To provide a reference for improving the quality standard of Schisandrae Sphenantherae Fructus,the ultra perform-ance liquid chromatography(UPLC)fingerprint of Schisandrae Sphenantherae Fructus and the contents of six lignans by a method of quantitative analysis of multi-components by single marker(QAMS)were established.The fingerprints of Schisan-drae Sphenantherae Fructus were established by UPLC and the UPLC fingerprints were analyzed by hierarchical cluster analy-sis(HCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)with schisantherin A as the internal refer-ence,QAMS was established for the determinations of schisantherin C,schisantherin B,anwuligan,schisandrin A and schisan-therin D,and compared with the results of external standard method(ESM)to determine the accuracy of the method.Fifteen common peaks were marked in the fingerprints of 14 batches of Schisandrae Sphenantherae Fructus,seven of which were iden-tified by comparison with reference substance,including protocatechuic acid,schisantherin C,schisantherin A,schisantherin B,anwuligan,schisandrin A,schisantherin D.The fingerprint similarities were above 0.95.Schisandrae Sphenantherae Fructus can be divided into four categories by HCA and OPLS-DA.The nine components,including peak 1(protocatechuic acid),peak 2,peak 4,peak 6,peak 7(schisantherin B),peak 8,peak 9,peak 12,and peak 15,were the main makers of the differ-ence in origin.There was no significant difference between the contents of schisantherin C,schisantherin B,anwuligan,schisantherin A,and schisantherin B determined by QAMS and ESM.The method is simple and reliable,providing a reference for the quality evaluation of Schisandrae Sphenantherae Fructus.
万方数据
维普
