Chemical constituents from flowers of Rhododendron pachypodum Balf.f.et W.W.Sm and their inhibitory activity on NO production
To study chemical constituents from the flowers of Rhododendron pachypodum Balf.f.et W.W.Sm and their anti-inflammatory activities.Systematic separation methods(silica gel column chromatography,gel Sephadex LH-20 column chro-matography,semi-preparative liquid chromatography)were used for the separation and purification of the ethyl acetate extract phase of R.pachypodum,and then the identification of compounds by modern spectroscopic methods,such as1H NMR,13C NMR,MS.The anti-inflammatory activities of compounds were evaluated by LPS induced RAW 264.7 inflammatory cell mod-el.Twenty six compounds were isolated from the EtOAc extracts of the flowers of this plant and identified as gray anotoxin Ⅰ(1),gray anotoxin Ⅱ(2),pieristoxin S(3),dehydrovomifoliol(4),megastigm-5-en-3,9-diol(5),vomifoliol(6),5,6-epox-y-3-hydroxy-7-megastigmen-9-one(7),hydroxy-3-oxo-α-ionol(8),geranyllinalool(9),negunfurol(10),2,6,10-trimeth-yldodeca-6,11-diene-2,3,10-triol(11),9,10-dihydroxy-6,10-dimethylundec-5-en-2-one(12),(2Z)-2,6-dimethyl-2,7-oc-tadiene-1,6-diol(13),medioresinol(14),pinoresinol(15),4-O-metbylcedrusin(16),naringenin(17),6,8-di-C-methyl-dihydrokaempferol(18),noreugenin(19),dihydroconiferyl alcohol(20),mycophenolic methyl ester(21),methyl orselli-nate(22),scopoletin(23),salicyl alcohol(24),p-methoxyphebylacetic acid(25),and 5-methylbenzene-1,3-diol(26).Compound 13,16 and 18 showed potential inhibitory activity on NO production.Compound 16 showed significant activity,the IC50 value of compound 16 was 33.5±1.5 μmol/L.
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