To study chemical constituents from the flowers of Rhododendron pachypodum Balf.f.et W.W.Sm and their anti-inflammatory activities.Systematic separation methods(silica gel column chromatography,gel Sephadex LH-20 column chro-matography,semi-preparative liquid chromatography)were used for the separation and purification of the ethyl acetate extract phase of R.pachypodum,and then the identification of compounds by modern spectroscopic methods,such as1H NMR,13C NMR,MS.The anti-inflammatory activities of compounds were evaluated by LPS induced RAW 264.7 inflammatory cell mod-el.Twenty six compounds were isolated from the EtOAc extracts of the flowers of this plant and identified as gray anotoxin Ⅰ(1),gray anotoxin Ⅱ(2),pieristoxin S(3),dehydrovomifoliol(4),megastigm-5-en-3,9-diol(5),vomifoliol(6),5,6-epox-y-3-hydroxy-7-megastigmen-9-one(7),hydroxy-3-oxo-α-ionol(8),geranyllinalool(9),negunfurol(10),2,6,10-trimeth-yldodeca-6,11-diene-2,3,10-triol(11),9,10-dihydroxy-6,10-dimethylundec-5-en-2-one(12),(2Z)-2,6-dimethyl-2,7-oc-tadiene-1,6-diol(13),medioresinol(14),pinoresinol(15),4-O-metbylcedrusin(16),naringenin(17),6,8-di-C-methyl-dihydrokaempferol(18),noreugenin(19),dihydroconiferyl alcohol(20),mycophenolic methyl ester(21),methyl orselli-nate(22),scopoletin(23),salicyl alcohol(24),p-methoxyphebylacetic acid(25),and 5-methylbenzene-1,3-diol(26).Compound 13,16 and 18 showed potential inhibitory activity on NO production.Compound 16 showed significant activity,the IC50 value of compound 16 was 33.5±1.5 μmol/L.
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