Study on thechemical constituents from the petroleum ether extract of Alocasia cucullata(Lour.)Schott and their breast cancer cell cytotoxicity
To study the chemical constituents of the petroleum ether extract from Alocasia cucullata(Lour.)Schott,a plant species belonging to the Araceae family.Silica gel,Sephadex LH-20 and other chromatographic methods were used for separation and purification,and spectroscopic techniques were employed for structural identification.The in vitro assessment of all isolated compounds was conducted using the CCK-8 assay on human breast cancer cell lines MDA-MB-231 and MCF-7,as well as mouse breast cancer cells 4T1.Fifteen compounds were isolated from the petroleum ether fraction of the rhizome of A.cucullata and their structures were identified as p-hydroxybenzaldehyde(1),benzoic acid(2),ethyl vanillate(3),ethyl gallate(4),7-hydroxycoumarin(5),myristate(6),squalene(7),oleanolic acid(8),ursolic acid(9),sclareol(10),sclareolide(11),protocatechualdehyde(12),protocatechuic acid(13),octacosanol(14)and gallic acid(15).Compounds 2-15were first isolated from genus Alocasia.All the compounds were first isolated from A.cucullata.The cytotoxic-ity study of breast cancer cell showed that compounds 4,8 and 9 had inhibitory effect on all three kinds of breast cancer cell lines.
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