To study the chemical constituents of the petroleum ether extract from Alocasia cucullata(Lour.)Schott,a plant species belonging to the Araceae family.Silica gel,Sephadex LH-20 and other chromatographic methods were used for separation and purification,and spectroscopic techniques were employed for structural identification.The in vitro assessment of all isolated compounds was conducted using the CCK-8 assay on human breast cancer cell lines MDA-MB-231 and MCF-7,as well as mouse breast cancer cells 4T1.Fifteen compounds were isolated from the petroleum ether fraction of the rhizome of A.cucullata and their structures were identified as p-hydroxybenzaldehyde(1),benzoic acid(2),ethyl vanillate(3),ethyl gallate(4),7-hydroxycoumarin(5),myristate(6),squalene(7),oleanolic acid(8),ursolic acid(9),sclareol(10),sclareolide(11),protocatechualdehyde(12),protocatechuic acid(13),octacosanol(14)and gallic acid(15).Compounds 2-15were first isolated from genus Alocasia.All the compounds were first isolated from A.cucullata.The cytotoxic-ity study of breast cancer cell showed that compounds 4,8 and 9 had inhibitory effect on all three kinds of breast cancer cell lines.
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