摘要
研究天南星科海芋属植物尖尾芋Alocasia cucullata(Lour.)Schott石油醚部位化学成分.采用硅胶、Sepha-dex LH-20等色谱方法进行分离纯化,运用波谱学技术进行结构鉴定.采用CCK-8法测试化合物对人乳腺癌MDA-MB-231、MCF-7细胞及小鼠乳腺癌4T1细胞的体外细胞毒活性.从尖尾芋根茎石油醚部位中分离得到15个化合物,分别为对羟基苯甲醛(1)、苯甲酸(2)、香草酸乙酯(3)、没食子酸乙酯(4)、7-羟基香豆素(5)、肉豆蔻酸甲酯(6)、角鲨烯(7)、齐墩果酸(8)、熊果酸(9)、香紫苏醇(10)、香紫苏内酯(11)、原儿茶醛(12)、原儿茶酸(13)、二十八烷醇(14)和没食子酸(15),化合物2～15为海芋属首次分离得到,所有化合物均为尖尾芋首次分离得到.乳腺癌细胞毒活性研究显示:化合物4、8和9对3种乳腺癌细胞均有一定的抑制作用.
Abstract
To study the chemical constituents of the petroleum ether extract from Alocasia cucullata(Lour.)Schott,a plant species belonging to the Araceae family.Silica gel,Sephadex LH-20 and other chromatographic methods were used for separation and purification,and spectroscopic techniques were employed for structural identification.The in vitro assessment of all isolated compounds was conducted using the CCK-8 assay on human breast cancer cell lines MDA-MB-231 and MCF-7,as well as mouse breast cancer cells 4T1.Fifteen compounds were isolated from the petroleum ether fraction of the rhizome of A.cucullata and their structures were identified as p-hydroxybenzaldehyde(1),benzoic acid(2),ethyl vanillate(3),ethyl gallate(4),7-hydroxycoumarin(5),myristate(6),squalene(7),oleanolic acid(8),ursolic acid(9),sclareol(10),sclareolide(11),protocatechualdehyde(12),protocatechuic acid(13),octacosanol(14)and gallic acid(15).Compounds 2-15were first isolated from genus Alocasia.All the compounds were first isolated from A.cucullata.The cytotoxic-ity study of breast cancer cell showed that compounds 4,8 and 9 had inhibitory effect on all three kinds of breast cancer cell lines.
基金项目
国家自然科学基金(81973593)
湖南省自然科学基金-科药联合项目(2022JJ80087)
湖南中医药大学研究生广东一方制药有限公司科研基金(2021YF01)
湖南中医药大学中药学一流学科基金(校行科字20183号)
维普
万方数据