Optimization of extraction process of Arctii Fructus polysaccharide based on response surface method and its anti-inflammatory effect on cells
The aim of this paper was to determine the optimum extraction conditions of Arctii Fructus polysaccharide and to explore the anti-inflammatory effect of its solution.The effects of different solid-liquid ratio,extraction time and extraction temperature on the extraction yield of Arctii Fructus polysaccharide were investigated by response surface method.The levels of tumor necrosis factor α(TNF-α)and interleukin-6(IL-6)in different RAW 264.7 cell treatment groups were determined by ELISA kit.The protein expression levels of myeloid differentiation factor 88(MyD88),Toll-like receptor 4(TLR4)and nuclear transcription factor-κB(NF-κB)were detected by Western blot.The results showed that the optimal extraction condi-tions were solid-liquid ratio 1∶15(g/mL),extraction time 3 h,extraction temperature 80℃,and the extraction yield was 7.19％.Arctii Fructus polysaccharide groups inhibited the secretion of TNF-α and IL-6,and played an immunoregulatory role by regulating the expression of proteins in related pathways.This study can lay a foundation for the development and applica-tion of new drug ingredients of Arctii Fructus.
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