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不同干燥方法对合欢花药材化学成分及其抗氧化活性影响

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建立测定合欢花药材中槲皮苷、异槲皮苷和槲皮素3种黄酮类成分含量测定的方法.通过化学成分含量及其抗氧化活性测定,探讨不同干燥方法对不同规格合欢花药材的影响,为合欢花药材的初加工工艺选择提供参考.通过晒干、阴干、蒸汽杀青后烘干、微波杀青后烘干、烘干(30、50、70、90 ℃)、冷冻干燥的方式对两种规格合欢花药材进行干燥处理,采用紫外分光光度法、高效液相色谱法、比色法和ABTS法、DPPH法等检测样品中总黄酮、异槲皮苷、槲皮苷、槲皮素含量及抗氧化活性.利用主成分分析、聚类分析和相关性分析对干燥后不同规格样品进行综合评价.研究结果表明,不同干燥处理合欢花药材样品化学成分和抗氧化活性结果存在显著差异.相关性分析表明,槲皮苷含量和总黄酮含量呈显著正相关(P<0.05),槲皮苷含量可作为总黄酮含量大小的评价指标,与O2-自由基清除能力呈正相关但不显著.槲皮苷含量和总黄酮含量与其他四种抗氧化指标呈显著正相关(P<0.05)或极显著正相关(P<0.01),槲皮素含量与ABTS自由基清除能力呈显著正相关(P<0.05),推测槲皮苷和槲皮素是2种合欢花类药材主要抗氧化活性物质.不同干燥方法对2种规格合欢花药材影响显著,盛开的合欢花适合宜采用70℃烘干处理,花蕾期的合欢米宜采用冷冻干燥处理,合欢花和合欢米均不宜采用阴干处理.本研究为2种合欢花类药材初加工方法的选择提供实验依据.
Effects of different drying methods on the chemical composition and antioxidant activity of Albiziae Flos
To establish a method for the determination of quercitrin,isoquercitrin and quercetin in Albiziae Flos.Through the determination of the contents of chemical components and their antioxidant activities,the effects of different drying methods on different specifications of Albiziae Flos were explored to provide a reference for the selection of the primary processing tech-nology of Albiziae Flos.The two specifications of Albiziae Flos were dried by sun-drying,shade-drying,drying after steam-kill-ing,drying after microwave-killing,hot-air drying(30,50,70,90 ℃),and freeze-drying,and the samples were tested for total flavonoids,isoquercitrin,quercitrin,quercetin content and and antioxidant activity by ultraviolet spectrophotometry(UV),high-performance liquid chromatography(HPLC),colorimetric method and ABTS,DPPH methods.The samples of different specifications after drying were comprehensively evaluated using principal component analysis(PCA),cluster analysis(CA)and correlation analysis.The results of the study show that,the results of chemical composition and antioxidant activity of sam-ples from different drying treatments were significantly different.Correlation analysis showed that the total flavonoid content and quercitrin content were significantly positively correlated(P<0.05),and quercitrin content could be used as an evalua-tion index for the magnitude of the total flavonoid content,quercitrin was positively but not significantly correlated with O2 radical scavenging capacity.Quercitrin content and total flavonoid content showed significant positive correlation(P<0.05)or highly significant positive correlation(P<0.01)with the other four antioxidant indices,and quercetin content was significantly and positively correlated with ABTS free radical scavenging capacity(P<0.05)and it was hypothesised that quercitrin and quercetin were the main antioxidant active substance in the two specifications of Albiziae Flos.Different drying methods had significant effects on the two specifications of Albiziae Flos,and it was suitable and appropriate to use 70 ℃ dr-ying treatment for the Albizia julibrissin Durazz.flower(AJF),and freeze-drying treatment for Albizia julibrissin Durazz.flower bud(AJFB),and it was not suitable to use shade-drying treatment for both AJF and AJFB.This study provides an experimen-tal basis for the selection of the primary processing methods of the two specifications of Albiziae Flos.

Albiziae Flosdrying methodschemical compositionantioxidant activity

左亚锋、徐秀泉、李巧月、王孟虎、孟祥松、闫攀、金传山、吴德玲

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亳州学院中药学院

亳州学院中药原料产品研发安徽普通高校重点实验室,亳州 236800

安徽中医药大学药学院,合肥 230012

合欢花 干燥方法 化学成分 抗氧化活性

安徽省高等学校自然科学研究重点项目安徽省高等学校自然科学研究重点项目亳州学院中药学团队项目安徽省高等学校学科(专业)拔尖人才培育项目

KJ2020A07652022AH052416BYZXKTD202004gxbjZD2020095

2024

天然产物研究与开发
中国科学院成都文献情报中心

天然产物研究与开发

CSTPCD北大核心
影响因子:0.783
ISSN:1001-6880
年,卷(期):2024.36(1)
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