Effects of different drying methods on the chemical composition and antioxidant activity of Albiziae Flos
To establish a method for the determination of quercitrin,isoquercitrin and quercetin in Albiziae Flos.Through the determination of the contents of chemical components and their antioxidant activities,the effects of different drying methods on different specifications of Albiziae Flos were explored to provide a reference for the selection of the primary processing tech-nology of Albiziae Flos.The two specifications of Albiziae Flos were dried by sun-drying,shade-drying,drying after steam-kill-ing,drying after microwave-killing,hot-air drying(30,50,70,90 ℃),and freeze-drying,and the samples were tested for total flavonoids,isoquercitrin,quercitrin,quercetin content and and antioxidant activity by ultraviolet spectrophotometry(UV),high-performance liquid chromatography(HPLC),colorimetric method and ABTS,DPPH methods.The samples of different specifications after drying were comprehensively evaluated using principal component analysis(PCA),cluster analysis(CA)and correlation analysis.The results of the study show that,the results of chemical composition and antioxidant activity of sam-ples from different drying treatments were significantly different.Correlation analysis showed that the total flavonoid content and quercitrin content were significantly positively correlated(P<0.05),and quercitrin content could be used as an evalua-tion index for the magnitude of the total flavonoid content,quercitrin was positively but not significantly correlated with O2 radical scavenging capacity.Quercitrin content and total flavonoid content showed significant positive correlation(P<0.05)or highly significant positive correlation(P<0.01)with the other four antioxidant indices,and quercetin content was significantly and positively correlated with ABTS free radical scavenging capacity(P<0.05)and it was hypothesised that quercitrin and quercetin were the main antioxidant active substance in the two specifications of Albiziae Flos.Different drying methods had significant effects on the two specifications of Albiziae Flos,and it was suitable and appropriate to use 70 ℃ dr-ying treatment for the Albizia julibrissin Durazz.flower(AJF),and freeze-drying treatment for Albizia julibrissin Durazz.flower bud(AJFB),and it was not suitable to use shade-drying treatment for both AJF and AJFB.This study provides an experimen-tal basis for the selection of the primary processing methods of the two specifications of Albiziae Flos.