Study on the mechanism of Tibetan medicine Myricaria germanica on ulcerative colitis
Based on network pharmacology,molecular dock and experimental verification,the effect and mechanism of Myri-caria germanica in the treatment of ulcerative colitis(UC)were investigated.The UHPLC-Q-Exactive-Orbitrap-MS technique was used for the rapid analysis of the original phytochemical components of M.germanica;the core components,target genes and main pathways of M.germanica for the treatment of UC were predicted and screened based on the network pharmacology,and molecular docking validation was carried out on the core components and targets;and the therapeutic effects of M.ger-manica on UC were observed by the mouse model of UC induced by dextran sulfate sodium(DSS).Twenty-three active ingre-dients of M.germanica for the treatment of UC were screened and acted on 111 common targets;PPI network analysis showed that AKT1,TNF,CASP3,etc.might be the core targets;GO biological process mainly involved inflammatory response,immune response,etc.;KEGG signaling pathway mainly involved MAPK signaling pathway,TNF signaling pathway,etc.;molecular docking showed that TNF,CASP3 and other core targets could bind well with the core active components;M.germanica can improve colon length and colonic mucosal damage in DSS-induced UC mice,and reduce the levels of TNF-α and IL-6 in colon and serum.M.germanica exerts its therapeutic effects on UC by modulating TNF-α,PTGS2 and other targets through com-pounds such as rheic acid,isorhamnetin,ellagic acid,wedelolactone and desmethylwedelolactone.
万方数据
维普
