天然产物研究与开发2024,Vol.36Issue(2) :218-226,267.DOI:10.16333/j.1001-6880.2024.2.004

不同品种柚子皮精油成分及抗氧化与抑菌能力对比研究

Comparison of components,antioxidant and bacteriostatic ability of different varieties of pomelo peel essential oil

肖新生 宋子龙 谢永燕 袁志辉
天然产物研究与开发2024,Vol.36Issue(2) :218-226,267.DOI:10.16333/j.1001-6880.2024.2.004

不同品种柚子皮精油成分及抗氧化与抑菌能力对比研究

Comparison of components,antioxidant and bacteriostatic ability of different varieties of pomelo peel essential oil

肖新生 1宋子龙 1谢永燕 1袁志辉1
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作者信息

  • 1. 湖南科技学院化学与生物工程学院,永州 425199
  • 折叠

摘要

柚子皮是一种富含精油的具有药用价值的生物质资源,但其精油成分与生物活性尚未深入研究.本文首先通过水蒸汽蒸馏法提取了 12个不同品种的柚子皮精油,接着用气相色谱质谱联用仪(GC-MS)对精油成分与相对含量进行了测定,然后采用DPPH自由基清除法、滤纸片法对不同品种柚子皮精油的抗氧化和抑菌能力进行比较,最后对不同品种柚子皮精油GC-MS分析数据进行主成分分析和聚类分析,结合抗氧化和抑菌实验结果,着力于理清柚子皮精油成分与抗氧化、抑菌能力的关系.实验结果表明:柚子皮精油的提取率为0.22%~1.52%;GC-MS法从不同品种柚子皮精油中鉴定出D-柠檬烯、β-月桂烯、α-蒎烯、α-水芹烯、芳樟醇等35种化学成分;DPPH自由基清除实验表明柚子皮精油的抗氧化能力与精油浓度呈正相关性;滤纸片法抑菌实验表明柚子皮精油有一定的抑菌效果,且对特定的菌种抑菌效果更明显.主成分分析和聚类分析结果表明,抗氧化和抑菌效果显著的成分有D-柠檬烯、柠檬醛、芳樟醇,而石竹烯、香叶烯醇、α-水芹烯也具有一定的抗氧化和抑菌能力.

Abstract

Pomelo peel is a kind of medicinal biomass resource rich in essential oil,but its essential oil composition and bio-logical activity have not been deeply studied.In this paper,12 different varieties of pomelo peelessential oils were extracted by steam distillation,and then the essential oil components and relative contents were determined by gas chromatography-mass spectrometry(GC-MS).Then the antioxidant and antibacterial activities of different varieties of pomelo peel essential oils were compared by DPPH radical scavenging method and filter paper method.Finally,principal component analysis and cluster analysis were performed on the GC-MS analysis data of different varieties of pomelo peel essential oils,and combined with the results of antioxidant and antibacterial experiments,the relationship between the components of pomelo peel essential oils and their antioxidant and antibacterial abilities was clarified.The results showed that the extraction rate of essential oil was 0.22%-1.52%.Thirty-five chemical components,such as D-limonene,β-laurene,α-pinene,α-aquinene and linalool,were i-dentified from different varieties of pomelo peel essential oil by GC-MS.DPPH scavenging experiment showed that the antioxi-dant capacity of pomelo peel essential oil was positively correlated with the concentration of essential oil.The experimental re-sults of filter paper showed that pomelo peel essential oil had a certain antibacterial effect,and the antibacterial effect was more obvious to specific strains.The results of principal component analysis and cluster analysis showed that D-limonene,cit-ral and linalool had significant antioxidant and bacteriostatic effects,while caryophyllene,geranylenol and α-hydroconene also had certain antioxidant and bacteriostatic abilities.

关键词

柚子皮精油/抗氧化能力/抑菌能力/气相色谱质谱联用仪

Key words

pomelo peel essential oil/antioxidant ability/antibacterial ability/GC-MS

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基金项目

湖南省自然科学基金面上项目(2021JJ30289)

湖南省自然科学基金面上项目(2023JJ50070)

出版年

2024
天然产物研究与开发
中国科学院成都文献情报中心

天然产物研究与开发

CSTPCD北大核心
影响因子:0.783
ISSN:1001-6880
参考文献量21
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