Study on the chemical constituents from viscera of Apostichopus japonicus
To study the chemical constituents of the viscera of Apostichopus japonicus,column chromatography,such as silica gel,ODS columns,semipreparative HPLC,and Sephadex LH-20 column chromatography,has been used to isolate the seconda-ry metabolites.Their structures were characterized on the basis of multiple spectrometric and spectroscopic techniques,inclu-ding 1H NMR,13C NMR,HSQC,HMBC,1H-1H COSY,NOESY,and HR-ESI-MS.As a result,fourteen compounds were isola-ted from the 95％ethanol extract of the viscera of A.japonicus.These compounds were identified as(E)-dec-5-en-1-yl sulfate(1),(Z)-dec-5-en-l-yl sulfate(2),7-methyloctyl sulfate(3),(S)-2,6-dimethylheptyl sulfate(4),N-lactoyl-phenylala-nine(5),(1S,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(6),(1R,3S)-1-methyl-1,2,3,4-tetra-hydro-β-carboline-3-carboxylic acid(7),L-phenylalanine(8),tyramine(9),tryptamine(10),L-tryptophan(11),8-oxo-tryptamine(12),3-(2-aminoethyl)-3-hydroxyindolin-2-one(13),β-carboline(14).Compound 1 was a novel compound.Compounds 5-14 were obtained from A.japonicus for the first time.
Apostichopus japonicusviscerachemical constituentsseparation and purificationstructure identification
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