Quality control of Puerariae Flos based on HPLC fingerprint and quantitative analysis of multi-components with single-marker method
To establish the HPLC fingerprint of Puerariae Flos and quantitative analysis of multi-components by single-marker(QAMS)for ten main flavonoids,in order to quality evaluation of Puerariae Flos in different regions.HPLC was used to ana-lyze the chemical components from Puerariae Flos.The chromatographic conditions were optimized,and the fingerprint was es-tablished.Kakkalide was used as the reference compound to establish QAMS method for the determination of the main effec-tive components in Puerariae Flos,and compared with the results of external standard method(ESM)to verify the accuracy of this method.The common mode for Pueraria lobata(Willd.)Ohwi(GH)and Pueraria thomsonii Benth.(FGH)fingerprint was established;the similarity were greater than 0.85 in 20 batches of GH and 10 batches of FGH;18 and 27 common finger-print peaks were identified.Kakkalide was used as the reference compound.The repeatability and durability of relative correc-tion factor for each component was good.There was no significant difference(P＞0.05)between the measured values of QAMS method and ESM,relative deviation＜3％.The components contents in Puerariae Flos from different origins were both greatly different.The results demonstrated quality control standard of Puerariae Flos should be improved.The establishment of fingerprint and QAMS method in this study was rapid,simple,objectively and effectively and full quality evaluation of Puerari-ae Flos,which can provide the references for the breeding and breeding high-quality germplasm resources and improving the quality control standard of Puerariae Flos when revising the Puerariae Flos.
万方数据
维普
