摘要
对房山紫堇Corydalis fangshanensis地上部分化学成分及其体外抗乳腺肿瘤细胞活性进行研究.采用各种色谱分离技术对乙酸乙酯和正丁醇两个萃取部位进行系统分离纯化,通过多种波谱数据(1D、2D NMR、MS、IR、UV)鉴定化合物结构,从房山紫堇乙酸乙酯和正丁醇两个部位中鉴定了 17个化合物,分别为7-acetyl thalifoline(1)、pycnar-rhine(2)、corynoxidine(3)、trans-isocorypalmine N-oxide(4)、四氢巴马汀(5)、四氢药根碱(6)、liriotulipiferine(7)、异波尔定碱(8)、乌药碱(9)、脱氢甲卡维丁(10)、feruloylputrescine(11)、木黄酮(12)、二甲基咖啡酸(13)、4-羟基-3-甲氧基桂皮酰基-β-D-葡萄糖苷(14)、水仙苷(15)、丁香脂素-4-O-β-D-葡萄糖苷(16)、异落叶松脂醇9-O-β-D-葡萄糖苷(17).其中化合物1作为天然产物首次报道,2～17是首次从房山紫堇中分离得到.结合体外MTT法评价单体化合物对人乳腺癌肿瘤细胞(MDA-MB-231、MCF-7)抑制作用.结果显示化合物2～10、13、15～17对MDA-MB-231细胞有一定的抑制作用,其IC50值范围为8.00～79.99 μmol/L,化合物2～10、16、17对MCF-7细胞有抑制作用,IC50值范围为 7.29～86.91 μmol/L.
Abstract
To investigate the chemical constituents from the aerial parts of Corydalis fangshanensis and the inhibitory effects on the human breast cancer cell lines(MCF-7 and MDA-MB-231).The chemical constituents of ethyl acetate and n-butanol ex-tracts were systematically isolated and purified by multiple column chromatography.The constituents were identified by NMR(1D,2D),MS,IR,UV,etc.Seventeen compounds were obtained and identified as 7-acetyl thalifoline(1),pycnarrhine(2),corynoxidine(3),trans-isocorypalmine N-oxide(4),tetrahydropalmatine(5),tetrahydrojatrorrhizine(6),liriotulipiferine(7),isoboldine(8),(R)-N-methylcoclaurine(9),dehydroapocavidine(10),feruloylputrescine(11),xylogranatinin(12),dimethylcaffic acid(13),4-hydroxy-3-methoxycinnamyl-β-D-glucopyranoside(14),narcissin(15),syringaresinol-4-O-β-D-glucopyranoside(16),isolariciresinol-9-O-β-D-glucopyranoside(17).Compound 1 was first reported as a natural product,2-17 were obtained from Corydalis fangshanensis for the first time.The inhibitory effects assay was carried out by MTT method.Anti-tumor activities assay indicated that 2-10,13,15-17 exhibited certain inhibition on MDA-MB-231 cell lines with the IC50 values of 8.00-79.99 μmol/L,and the IC50 values of 2-10,16,17 for the MCF-7 cell lines were 7.29-86.91μmol/L.
基金项目
宁夏回族自治区自然科学基金一般项目(2022AAC03292)
北方民族大学科研启动基金(2021KYQD35)
山西省自然科学基金青年基金(201801D221432)
山西中医药大学科技创新能力培育计划(2019PY-072)
维普
万方数据