Chemical constituents from the aerial parts of Corydalis fangshanensis and their inhibitory effects on breast cancer cells proliferation
To investigate the chemical constituents from the aerial parts of Corydalis fangshanensis and the inhibitory effects on the human breast cancer cell lines(MCF-7 and MDA-MB-231).The chemical constituents of ethyl acetate and n-butanol ex-tracts were systematically isolated and purified by multiple column chromatography.The constituents were identified by NMR(1D,2D),MS,IR,UV,etc.Seventeen compounds were obtained and identified as 7-acetyl thalifoline(1),pycnarrhine(2),corynoxidine(3),trans-isocorypalmine N-oxide(4),tetrahydropalmatine(5),tetrahydrojatrorrhizine(6),liriotulipiferine(7),isoboldine(8),(R)-N-methylcoclaurine(9),dehydroapocavidine(10),feruloylputrescine(11),xylogranatinin(12),dimethylcaffic acid(13),4-hydroxy-3-methoxycinnamyl-β-D-glucopyranoside(14),narcissin(15),syringaresinol-4-O-β-D-glucopyranoside(16),isolariciresinol-9-O-β-D-glucopyranoside(17).Compound 1 was first reported as a natural product,2-17 were obtained from Corydalis fangshanensis for the first time.The inhibitory effects assay was carried out by MTT method.Anti-tumor activities assay indicated that 2-10,13,15-17 exhibited certain inhibition on MDA-MB-231 cell lines with the IC50 values of 8.00-79.99 μmol/L,and the IC50 values of 2-10,16,17 for the MCF-7 cell lines were 7.29-86.91μmol/L.
Corydalis fangshanensischemical componentsstructure elucidationbreast cancer inhibitory activity
万方数据
维普
