To investigate the chemical constituents from the aerial parts of Corydalis fangshanensis and the inhibitory effects on the human breast cancer cell lines(MCF-7 and MDA-MB-231).The chemical constituents of ethyl acetate and n-butanol ex-tracts were systematically isolated and purified by multiple column chromatography.The constituents were identified by NMR(1D,2D),MS,IR,UV,etc.Seventeen compounds were obtained and identified as 7-acetyl thalifoline(1),pycnarrhine(2),corynoxidine(3),trans-isocorypalmine N-oxide(4),tetrahydropalmatine(5),tetrahydrojatrorrhizine(6),liriotulipiferine(7),isoboldine(8),(R)-N-methylcoclaurine(9),dehydroapocavidine(10),feruloylputrescine(11),xylogranatinin(12),dimethylcaffic acid(13),4-hydroxy-3-methoxycinnamyl-β-D-glucopyranoside(14),narcissin(15),syringaresinol-4-O-β-D-glucopyranoside(16),isolariciresinol-9-O-β-D-glucopyranoside(17).Compound 1 was first reported as a natural product,2-17 were obtained from Corydalis fangshanensis for the first time.The inhibitory effects assay was carried out by MTT method.Anti-tumor activities assay indicated that 2-10,13,15-17 exhibited certain inhibition on MDA-MB-231 cell lines with the IC50 values of 8.00-79.99 μmol/L,and the IC50 values of 2-10,16,17 for the MCF-7 cell lines were 7.29-86.91μmol/L.
Corydalis fangshanensischemical componentsstructure elucidationbreast cancer inhibitory activity
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